Browsing Linhardt Research Labs Papers by Title
Now showing items 508-527 of 1022
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Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization
(2017-01-06)Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin. Based on the types of processing reactions and the structures of the products, ... -
Hyphenated techniques for the analysis of heparin and heparan sulfate
(Springer, 2011-01-01)The elucidation of the structure of glycosaminoglycan has proven to be challenging for analytical chemists. Molecules of glycosaminoglycan have a high negative charge and are polydisperse and microheterogeneous, thus ... -
Identification of a Heparin Binding Peptide on the ExtracellularDomain of the KDR VEGF Receptor
(1997)Vascular endothelial growth factor (VEGF), a potent and specific activator of endothelial cells, is expressed as multiple homodimeric forms resulting from alternative RNA splicing. VEGF121 does not bind heparin while the ... -
Identification of a novel structure in heparin generated by potassium permanganate oxidation
(Elsevier, 2010-10-15)The worldwide heparin contamination crisis in 2008 led health authorities to take fundamental steps to better control heparin manufacture, including implementing appropriate analytical and bio-analytical methods to ensure ... -
Identification of Keratan Sulfate Disaccharide Unit at the C-3 position of Glucuronic Acid of Chondroitin Sulfate from Mactra chinensis
(Portland Press Limited, 2016)Glycosaminoglycans (GAGs), including chondroitin sulfate (CS), dermatan sulfate, heparin, heparan sulfate and keratan sulfate (KS) are linear sulfated repeating disaccharide sequences containing hexosamine and uronic acid ... -
Identification of the Capsular Polysaccharides of Type D and F Pasteurella multocida as Unmodified Heparin and Chondroitin, Respectively
(2002-09-27)Pasteurella multocida is a pathogenic Gram-negative bacterial species that infects a wide variety of animals and humans. A notable morphological feature of many isolates is the extracellular capsule. The ability to remove ... -
Identification of the Streptococcus pneumoniae type 3 capsule-specific glycosyl hydrolase gene of Paenibacillus sp. 32352
(2018)Bacillus circulans Jordan 32352 was isolated from decaying organic matter in the New Jersey soil in the early 1930s. This soil-dwelling bacterium produced an enzyme capable of degrading the type 3 capsular polysaccharide ... -
Identification, Repair and Characterization of a Benzyl Alcohol-Inducible Promoter for Recombinant Proteins Overexpression in Corynebacterium glutamicum
(2020-11-01)Corynebacterium glutamicum is an important industrial organism for the production of a variety of biological commodities. We discovered a promoter encoded by the gene NCgl2319 in C. glutamicum, which could be induced by ... -
Immobilization of C3b with Retention of Functional Activity
(1990-10-04)We compared eight commercially available, pre-activated affinity chromatography supports for ability to immobilize C3b that would retain functional activity. Pre-activated supports that we studied were: cyanogen bromide ... -
Immobilization of Heparin-Approaches and Applications
(Bentham Science, 2008-01-01)Heparin, an anticoagulant, has been used in many forms to treat various diseases. These forms include soluble heparin and heparin immobilized to supporting matrices by physical adsorption, by covalent chemical methods and ... -
Immobilized enzymes to convert N-sulfo, N-acetyl heparosan to a critical intermediate in the production of bioengineered heparin
(Elsevier, 2013-09-10)Heparin is a critically important anticoagulant drug that is prepared from pig intestine. In 2007-2008, there was a crisis in the heparin market when the raw material was adulterated with the toxic polysaccharide, oversulfated ... -
Immobilized Heparinase: Productions, Purification, and Application In Extracorporeal Therapy
(1982-01-01)There are nearly 20,000,000 perfusions involving extracorporeal medical machines performed each year (1,2). The artificial kidney is employed several times weekly by over 100,000 persons each year (1). Open-heart operations ... -
Immuno-Affinity Purification of Heparinase
(1985-01-01)Polyclonal IgG rabbit antibodies were prepared against a purified heparinase from Flavobacterium heparinum. Immuno-affinity purification of crude and partially purified heparinase is described. The resulting enzyme was of ... -
Immunological Activity of Chondroitin Sulfate
(Elsevier, 2006-12-01)The use of chondroitin sulfate (CS) for the symptomatic treatment of osteoarthritis (OA) has become very popular; however, it has also been the subject of controversy for several reasons. First, the nutraceutical industry ... -
Impact of autoclave sterilization on the activity and structure of formulated heparin
(Elsevier, 2011-01-01)The stability of a formulated heparin was examined during its sterilization by autoclaving. A new method to follow loss in heparin binding to the serine protease inhibitor, antithrombin III, and the serine protease, thrombin, ... -
Impact of degree of oxidation on the physicochemical properties of microcrystalline cellulose
(2017-01-02)Microcrystalline cellulose, a major component of cell wall of plants, is one of the most abundant natural materials, but the poor solubility of cellulose limits its applications. Cellulose is a linear glucan with exclusive ... -
Impact of GFP tagging on Robo1-heparin interaction
(2014-01-01)Green fluorescent proteins (GFPs) and their derivatives are widely used as markers to visualize cells, protein localizations in in vitro and in vivo studies. The use of GFP fusion protein for visualization is generally ... -
Impact of high salt diet on N-acetylgalactosamine-4-sulfatase activity, glycosaminoglycans, and kininogen in rat kidney
(Springer, 2013)N-acetylgalactosamine-4-sulfatase (Arylsulfatase B; ARSB) is the enzyme that removes sulfate groups from the N-acetylgalactosamine-4-sulfate residue at the non-reducing end of chondroitin-4-sulfate (C4S) and dermatan sulfate ... -
Impact of hydrolysis conditions on detection of mannuronic to guluronic acids ratio in alginate and its derivatives
(2015-05-20)Alginate is a linear and acidic polysaccharide, composed of (1 → 4) linked β-D-mannuronic acid (ManA) and α-L-guluronic acid (GulA). The ratio of ManA to GulA (M/G) is one of the most important factors for the application ... -
Impact of temperature on heparin and protein interactions
(Omics, 2018)Heparin has many important biological activities, associated with a diverse set of interactions with biologically functional proteins. The binding mechanisms and biological significance of heparin-protein interactions have ...