Browsing Linhardt Research Labs Papers by Title
Now showing items 614-633 of 1022
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Mapping and Sequencing of Oligosaccharides by Electrophoresis
(1993-02-01)The analysis of oligosaccharides and polysaccharides takes on increased importance with the discovery of each new biological activity attributable to this important class of biopolymers. Our understanding of other biopolymers, ... -
Mapping the Structural and Dynamic Determinants of pH-sensitive Heparin Binding to Granulocyte Macrophage-colony Stimulating Factor
(American Chemical Society (ACS), 2020-09-29)Granulocyte macrophage colony stimulating factor (GMCSF) is an immunomodulatory cytokine that is harnessed as a therapeutic. GMCSF is known to interact with other clinically important molecules, such as heparin, suggesting ... -
Masquerading microbial pathogens: Capsular polysaccharides mimic host-tissue molecules
(2014-01-01)The increasing prevalence of antibiotic-resistant bacteria portends an impending postantibiotic age, characterized by diminishing efficacy of common antibiotics and routine application of multifaceted, complementary ... -
Mass balance analysis of contaminated heparin product
(Elsevier, 2011-01-01)A quantitative analysis of a recalled contaminated lot of heparin sodium injection U.S. Pharmacopeia (USP) was undertaken in response to the controversy regarding the exact nature of the contaminant involved in the heparin ... -
Mass spectrometric evidence for the mechanism of free-radical depolymerization of various types of glycosaminoglycans
(2020-04-01)Glycosaminoglycans (GAGs) are large, complex carbohydrate molecules that interact with a wide range of proteins involved in physiological and pathological processes. Several naturally derived GAGs have emerged as potentially ... -
Mass Spectrometry for the Analysis of Highly Charged Sulfated Carbohydrates
(Bentham Science, 2005)Sulfated polysaccharides and their oligosaccharide components play an important biological role in physiological and pathophysiological processes. Glycosaminoglycans, such as heparin, are currently used as pharmaceutical ... -
Mass spectrometry for the characterization of unsulfated chondroitin oligosaccharides from 2-mers to 16-mers. Comparison with hyaluronic acid oligomers
(Wiley, 2008-11-30)This study reports for the first time the complete liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) and tandem mass spectrometry (MS/MS) analyses performed in negative ion mode of saturated ... -
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Uncomplexed Highly Sulfated Oligosaccharides using Ionic Liquid Matrices
(American Chemical Society (ACS), 2006-03-15)Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under ... -
Measurement of the Antithrombin III Binding Site in Low Molecular Weight Heparins Using 13C NMR and Capillary Electrophoresis
(1995-01-01)Low molecular weight heparins, prepared from the controlled chemical or enzymatic depolymerization of the heparin polysaccharide, are currently replacing heparin as the clinical anticoagulant/antithrombotic agent of choice. ... -
Mechanism of Decomposition for Diacyl Peroxides and Related Carboxy Inversion Compounds in Aqueous Acetone
(Elsevier, 1979)The recent report of CINDP during the decomposition of t-butylacetyl m-chlorobenzoyl peroxide, which was interpreted as arising from polarized, rearranged ion pair intermediates, [1] prompts us to report the behavior of ... -
Mechanism of Diacyl Peroxide Decomposition
(1982)The presence of ion-pair intermediates in diacyl peroxide decomposition has been established. Various substituted (4-X·pOOiyl)phenylaoetyl Y·benzoyl peroxides and three corresponding 4·X-bemhydryl-4·nitrobenzolc carbonic ... -
The Mechanism of Diacyl Peroxide Decomposition
(Johns Hopkins University, 1979)Various substituted 4-X-diphenylacetyl Y-benzoyl peroxides have been prepared and characterized in situ by low temperature NMR and IR spectroscopy. These peroxides decompose in non-nucleophilic solvents below -20°C, with ... -
Mechanism of enhanced oral absorption of Akebia saponin D by a self-nanoemulsifying drug delivery system loaded with phospholipid complex
(2019-01-02)Akebia saponin D (ASD) exhibits a variety of pharmacological activities, such as anti-osteoporosis, neuroprotection, hepatoprotection, but has poor oral bioavailability. A self-nanoemulsifying drug delivery system loaded ... -
Mechanistic insights into the effect of imidazolium ionic liquids on the lipid production by Geotrichum fermentans
(Springer Nature, 2016-12-16)Background: Ionic liquid (IL) pretreatment has emerged as a promising technique that enables complete utilization of lignocellulosic biomass for biofuel production. However, imidazolium IL has recently been shown to exhibit ... -
Metabolic alteration of the N-glycan structure of a protein from patients with a heterozygous protein deficiency
(Elsevier, 2004-12-24)Glycosylation, an important post-translation modification, could alter biological activity or influence the clearance rates of glycoproteins. We report here the first example of a heterozygous protein deficiency leading ... -
Metabolic bioengineering: Glycans and glycoconjugates
(2018-10-01)The application of metabolic engineering to the production of glycans and glycoconjugates is the subject of this of Emerging Topics in Life Science. The lack of availability of these complex carbohydrate or saccharide ... -
Metabolic engineering and in vitro biosynthesis of natural and non-natural analogues of plant metabolites
(2013)Over the years, natural products from plants and their non-natural derivatives have shown to be active against different types of chronic diseases. However, isolation of such natural products can be limited due to their ... -
Metabolic Engineering of Bacillus megaterium for Heparosan Biosynthesis using Pasteurella multocida Heparosan Synthase, PmHS2
(Springer Nature, 2019-08-12)Background: Heparosan is the unsulfated precursor of heparin and heparan sulfate and its synthesis is typically the first step in the production of bioengineered heparin. In addition to its utility as the starting material ... -
Metabolic engineering of capsular polysaccharides
(2018-10-01)With rising concerns about sustainable practices, environmental complications, and declining resources, metabolic engineers are transforming microorganisms into cellular factories for producing capsular polysaccharides ... -
Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin
(Elsevier, 2012-03-01)Heparin is the most widely used pharmaceutical to control blood coagulation in modern medicine. A health crisis that took place in 2008 led to a demand for production of heparin from non-animal sources. Chinese hamster ...