Browsing Linhardt Research Labs Papers by Title
Now showing items 640-659 of 1022
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Microbially Produced Rhamnolipid as a Source of Rhamnose
(1989-01-01)The sugar L-rhamnose (6-deoxy-~-mannose) is used as a fine chemical in scientific and industrial settings, as a component in chemical reactions, and as a starting material in the synthesis of organic compounds. Present ... -
Microheterogeneity of Plasma Glycoproteins Antithrombin III and Heparin Cofactor II and Their Carbohydrate Analysis
(1988-07-01)Antithrombin III is the most important protease inhibitor in the blood coagulation cascade. Its activity results from the formation of covalent complexes with Factor Xa and thrombin (FIIa) (1). The rate of this inhibition ... -
Microscale Isolation and Analysis of Heparin from Plasma using an Anion Exchange Spin Column
(Elsevier, 2006-06-15)Heparin, a linear highly sulfated polysaccharide, is a member of the glycosaminoglycan (GAG)1 family [1,2]. Since the 1930s, heparin has been widely used as a clinical anticoagulant [3,4]. More recently, low-molecular ... -
Microscale separation of heparosan, heparan sulfate and heparin
(Elsevier, 2013-03-15)The separation and quantification of glycosaminoglycan (GAG) chains, with different levels of sulfation, from cells, media and prepared through chemoenzymatic synthesis or metabolic engineering, poses a major challenge in ... -
Mode of Action of Heparin Lyase On Heparin
(1982-04-03)Heparinase (heparin lyase, EC 4.2.2.7) prepared from Flavobacterium heparinum was used to digest heparin. The products of digestion were examined with a viscosometric assay at various stages of the reaction to measure their ... -
Modification and Potential Uses of Heparin and Other Acidic Polysaccharides
(1990)Heparin lo a polydisperse, highly sulfated, linear polysaccharide of repeating uronic acid and glucosamine residues (Figure 1). Although heperin has been used clinically as an anticoagulant for the past 5O years, its precise ... -
Modification of solid phase red cell adherence assay for the detection of platelet alloantibodies in patients with thrombocytopenia
(Oxford, 2008)Platelet refractoriness is caused by HLA antibodies and platelet-specific antibodies. Current methods used to detect antiplatelet antibodies have limitations. Solid phase red cell adherence (SPRCA) lacks sensitivity and ... -
Modified Assay for the Measurement of Biotin in the Presence of Heparin
(1995-01-01)Tween 20 is frequently used in blotting analysis of proteins and nucleic acids to decrease background staining due to nonspecific binding of antibodies to various membranes. Inclusion of 0.1% (w/v) Tween 20 in the washing ... -
Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells
(2015-03-18)Sodium butyrate, a histone deacetylase inhibitor, has been used to improve transgene expression in Chinese hamster ovary (CHO) cells. The current study explores the impact of butyrate treatment on heparan sulfate (HS) ... -
Molecular and biochemical profiling of a heparin-derived oligosaccharide, C3
(2002-02-15)This study was designed to characterize a heparin-derived oligosaccharide (HDO), C3, using chemical and biochemical methods. Although previous studies have suggested C3 as a promising compound in the treatment of Alzheimer's ... -
Molecular Mechanism of Host Specificity in Plasmodium Falciparum Infection: Role of Circumsporozoite Protein
(Elsevier, 2003-10-17)Plasmodium falciparum sporozoites invade liver cells in humans and set the stage for malaria infection. Circumsporozoite protein (CSP), a predominant surface antigen on sporozoite surface, has been associated with the ... -
Molecular mechanisms of bioactive polysaccharides from Ganoderma lucidum (Lingzhi), a review
(2020)Ganoderma lucidum, commonly known as "Lingzhi" in Chinese, are well-known medicinal mushrooms. Lingzhi has been used in traditional Chinese herbal medicines for more than two thousand years. G. lucidum polysaccharides ... -
Molecular size is important for the safety and selective inhibition of intrinsic factor Xase for fucosylated chondroitin sulfate
(2017-12-15)Fucosylated chondroitin sulfate from sea cucumber Isostichopus badionotus (FCS-Ib) showed potent anticoagulant activities without selectivity. The present study focused on developing safe FCS-Ib oligomers showing selective ... -
Molecular weight characterization for glycosaminoglycans with different degrees of sulfation by size exclusion chromatography
(Bentham Science, 2012)Different degrees of glycosaminoglycan sulfation result in their different charge densities. The charge density differences impact their migration behavior in polyacrylamide gel electrophoresis and size exclusion chromatography, ... -
Molecular Weight Dependent Tissue Factor Pathway Inhibitor Release by Heparin and Heparin Oligosaccharides
(Elsevier, 2007-02-28)Heparin and low molecular weight heparins exert their vascular effects by mobilizing tissue factor pathway inhibitor (TFPI) from the vascular endothelium into the blood circulation. We compared the influence of molecular ... -
Molecular Weight of Heparin Using 13C Nuclear Magnetic Resonance Spectroscopy
(1995-01-01)Heparin is a polydisperse, heterogeneous polysaccharide that has been used as an anticoagulant for the past 50 years. The molecular weight determination of this important drug has traditionally relied on gel permeation ... -
Molecular Weight of Low Molecular Heparins by 13C NMR Spectroscopy
(1994)Heparin and low molecular weight heparins are polydisperse polysaccharides with a degree of polymerization ranging from 4 to approximately 40. The determination of their average molecular weights has traditionally relied ... -
Monoclonal Antibodies Prepared Against Heparin Lyase I and their Reactivity Towards Heparin Lyases I, II and III
(1993)1. Six different monoclonal IgG mouse antibodies to heparin lyase I from Flavobacterium heparinum were prepared. 2. The monoclonal antibodies were used to detect heparin lyases I, II and III by dot-blotting immunoassay and ... -
Mosquito Heparan Sulfate and its Potential Role in Malaria Infection and Transmission
(Elsevier, 2007-08-31)Heparan sulfate has been isolated for the first time from the mosquito Anopheles stephensi, a known vector for Plasmodium parasites, the causative agents of malaria. Chondroitin sulfate, but not dermatan sulfate or hyaluronan, ... -
Mulberry: A review of bioactive compounds and advanced processing technology
(2019-01-01)Background: Historically, mulberry has been effectively used as a traditional medicine in Asia for the treatment of various infectious and internal diseases. It is a rich source of bioactive compounds that can promote human ...