Browsing Linhardt Research Labs Papers by Title
Now showing items 653-672 of 1022
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Molecular weight characterization for glycosaminoglycans with different degrees of sulfation by size exclusion chromatography
(Bentham Science, 2012)Different degrees of glycosaminoglycan sulfation result in their different charge densities. The charge density differences impact their migration behavior in polyacrylamide gel electrophoresis and size exclusion chromatography, ... -
Molecular Weight Dependent Tissue Factor Pathway Inhibitor Release by Heparin and Heparin Oligosaccharides
(Elsevier, 2007-02-28)Heparin and low molecular weight heparins exert their vascular effects by mobilizing tissue factor pathway inhibitor (TFPI) from the vascular endothelium into the blood circulation. We compared the influence of molecular ... -
Molecular Weight of Heparin Using 13C Nuclear Magnetic Resonance Spectroscopy
(1995-01-01)Heparin is a polydisperse, heterogeneous polysaccharide that has been used as an anticoagulant for the past 50 years. The molecular weight determination of this important drug has traditionally relied on gel permeation ... -
Molecular Weight of Low Molecular Heparins by 13C NMR Spectroscopy
(1994)Heparin and low molecular weight heparins are polydisperse polysaccharides with a degree of polymerization ranging from 4 to approximately 40. The determination of their average molecular weights has traditionally relied ... -
Monoclonal Antibodies Prepared Against Heparin Lyase I and their Reactivity Towards Heparin Lyases I, II and III
(1993)1. Six different monoclonal IgG mouse antibodies to heparin lyase I from Flavobacterium heparinum were prepared. 2. The monoclonal antibodies were used to detect heparin lyases I, II and III by dot-blotting immunoassay and ... -
Mosquito Heparan Sulfate and its Potential Role in Malaria Infection and Transmission
(Elsevier, 2007-08-31)Heparan sulfate has been isolated for the first time from the mosquito Anopheles stephensi, a known vector for Plasmodium parasites, the causative agents of malaria. Chondroitin sulfate, but not dermatan sulfate or hyaluronan, ... -
Mulberry: A review of bioactive compounds and advanced processing technology
(2019-01-01)Background: Historically, mulberry has been effectively used as a traditional medicine in Asia for the treatment of various infectious and internal diseases. It is a rich source of bioactive compounds that can promote human ... -
N,N,N-trimethyl chitosan nanoparticles for the delivery of monoclonal antibodies against hepatocellular carcinoma cells
(Elsevier, 2011-04-22)N,N,N-Trimethyl chitosan chloride is capable of forming nanocomplexes with protein through ionotropic gelation. A monoclonal antibody, raised against human liver heparan sulfate proteoglycan and specifically inhibiting ... -
N-glycans released from glycoproteins using a commercial kit and comprehensively analyzed with a hypothetical database
(Royal Society of Chemistry, 2017)Heparan sulfate (HS) is a member of the glycosaminoglycans (GAG) family that plays essential roles in biological processes from animal sources. Heparin, a highly sulfated form of HS, is widely used as anticoagulant drug ... -
N-glycolyl chondroitin synthesis using metabolically engineered E. coli
(Springer, 2020)N-glycolyl chondroitin (Gc-CN) is a metabolite of N-glycolylneuraminic acid (Neu5Gc), a sialic acid that is commonly found in mammals, but not humans. Humans can incorporate exogenous Neu5Gc into their tissues from eating ... -
N-sulfotestosteronan, a novel substrate for heparan sulfate 6-O-sulfotransferases and its analysis by oxidative degradation
(Wiley, 2013-10-01)Testosteronan, an unusual glycosaminoglycan (GAG) first isolated from the microbe Comamonas testosteroni, was enzymatically synthesized in vitro by transferring uridine diphosphate sugars on β-p-nitrophenyl glucuronide ... -
Nano-based Neoproteoglycans – Blood Compatible Carbon Nanotubes
(American Chemical Society (ACS), 2006-04-11)Although nanotechnology has provided a rich variety of nanomaterials (1–100 nm) for in vivo medical applications, the blood compatibility of all these nanobiomaterials is still largely unexamined. Here, we report the ... -
Nanostructured glycan architecture is important in the inhibition of influenza A virus infection
(2017-01-10)Rapid change and zoonotic transmission to humans have enhanced the virulence of the influenza A virus (IAV)3. Neutralizing antibodies fail to provide lasting protection from seasonal epidemics1,4. Furthermore, the effectiveness ... -
Naringenin-Responsive Riboswitch-Based Fluorescent Biosensor Module for Escherichia coli Co-Cultures
(2017-10-01)The ability to design and construct combinatorial synthetic metabolic pathways has far exceeded our capacity for efficient screening and selection of the resulting microbial strains. The need for high-throughput rapid ... -
Nature of the Interaction of Heparin with Acidic Fibroblast Growth Factor
(1993-01-01)The binding of human acidic fibroblast growth factor (aFGF) to heparin has been analyzed by a variety of different approaches to better elucidate the nature of this protein/sulfated polysaccharide interaction. Static and ... -
Negative electron transfer dissociation Fourier transform mass spectrometry of glycosaminoglycan carbohydrates
(Sage, 2011-01-01)Electron transfer through gas-phase ion–ion reactions has led to the widespread application ofelectron-based techniques once only capable in ion trapping mass spectrometers. Although anymass analyzer can, in theory, be ... -
Negative electron transfer dissociation of glycosaminoglycans
(American Chemical Society (ACS), 2010)Structural characterization of glycosaminoglycans (GAGs) has been a challenge in the field of mass spectrometry, and the application of electron detachment dissociation (EDD) Fourier transform ion cyclotron resonance mass ... -
Negative-Ion Fast Atom Bombardment Tandem Mass Spectrometry to Determine Sulfate and Linkage Position in Glycosaminoglycan-Derived Disaccharides
(1992-01-01)Negative ion fast-atom bombardment tandem mass spectrometry has been used in the analysis of monosulfated. disaccharides. These commercially obtained disaccharides have been enzymatically prepared from glycosaminoglycans ... -
Negative-ion mode capillary isoelectric focusing-mass spectrometry for charge-based separation of acidic oligosaccharides
(2019-01-02)Glycosaminoglycans (GAGs) are biologically and pharmacologically important linear, anionic polysaccharides containing various repeating disaccharides sequences. The analysis of these polysaccharides generally relies on ... -
Neoproteoglycans in tissue engineering
(FEBS Press, 2013-05-01)Proteoglycans, comprised of a core protein to which glycosaminoglycan chains are covalently linked, are an important structural and functional family of macromolecules found in the extracellular matrix. Advances in our ...