Browsing Linhardt Research Labs Papers by Title
Now showing items 680-699 of 1022
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New Ionic Liquid Matrix for Direct UV-MALDI-TOF-MS Analysis of Dermatan Sulfate and Chondroitin Sulfate Oligosaccharides
(American Chemical Society (ACS), 2007)Polyanionic oligosaccharides such as dermatan sulfate (DS) and chondroitin sulfate (CS) exhibit poor ionization efficiencies and tend to undergo thermal fragmentation through the loss of SO(3) under conventional ultraviolet ... -
New Methodologies in Heparin Structure Analysis and the Generation of Low Molecular Weight Heparins
(1992)This chapter examines the methods used to prepare heparin, low molecular weight (LMW) heparins, and immobilized heparins. These heparins and heparin derivatives arc polydispcrse heterogeneous mixtures that require structural ... -
New Potent Insecticidal Agent: 4’-Fucosyl Avermectin Derivative
(2004-09-06)A 4′-fucosyl avermectin derivative was designed and synthesized. This new avermectin derivative showed excellent in vivo bioactivity against cabbage larvae when compared to commercially available avermectin B1a. In this ... -
NMR quantification for monitoring heparosan K5 capsular polysaccharide production
(Elsevier, 2010)Traditional chromatographic quantification methods for heparosan produced from the Escherichia coli K5 strain rely on extensive purification requiring laborious sample preparation. These methods are time-consuming, often ... -
NMR Solution Conformation of Heparin-Derived Hexasaccharide
(1997-11-15)The solution conformation of homogeneous, heparin-derived hexasaccharide (residues A, B, C, D, E, F) has been investigated by using 1H-NMR spectroscopy. Intra-ring conformations have been defined by J-coupling constants ... -
NMR Solution Conformation of Heparin-Derived Tetrasaccharide
(1996-08-15)The solution conformation of the homogeneous, heparin-derived tetrasaccharide delta UA2S(1-->4)-alpha-D-GlcNpS6S(1-->4)-alpha-L-IdoAp2S (1-->4)-alpha-D-GlcNpS6S (residues A, B, C and D respectively, where IdoA is iduronic ... -
Non-anticoagulant heparin as a pre-exposure prophylaxis prevents Lyme disease infection
(American Chemical Society (ACS), 2020-03-13)Lyme disease (LD) is caused by the spirochete Borrelia burgdorferi sensu lato (Bbsl). After transmission to humans by ticks, Bbsl spreads to multiple organs, leading to arthritis, carditis, and neuroborreliosis. No effective ... -
Non-anticoagulant low molecular weight heparins for pharmaceutical applications
(2019-01-24)Heparin is a polypharmacological agent with anticoagulant activity. Periodate oxidation of the nonsulfated glucuronic acid residue results in non-anticoagulant heparin derivative (NACH) of reduced molecular weight. Similar ... -
Non-Random Structural Features in the Heparin Polymer
(1989)Computer simulation studies were used to prepare an ensemble of heparin number chains. The polydispersity of these chains was simulated by introducing a specific "fraction of terminators", and it closely resembled the ... -
Novel Cellulose – Halloysite Hemostatic Nanocomposite Fibers with a Dramatic Reduction in Human Plasma Coagulation Time
(2019-05-01)High-performance cellulose–halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet–wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin ... -
Novel interactions of glycosaminoglycans and bacterial glycolipids mediate binding of enterococci to human cells
(Elsevier, 2009-07-03)Enterococcus faecalis is among the most important nosocomial pathogens. The intestinal mucosa is considered to be the main site used by these bacteria for entrance and dissemination. A better understanding of the mechanisms ... -
Novel Method for Measurement of Heparin Anticoagulant Activity Using SPR
(Elsevier, 2017-06-01)A novel method has been developed for the easy measurement of heparin's anticoagulant activity using surface plasmon resonance. The anticoagulant activity of target heparin was evaluated by measuring the competitive ... -
Nucleolin: Acharan Sulfate-Binding Protein on the Surface of Cancer Cells
(Oxford, 2005-01-01)Glycosaminoglycans (GAGs) are complex polysaccharides that participate in the regulation of physiological processes through the interactions with a wide variety of proteins. Acharan sulfate (AS), isolated from the giant ... -
Oligosaccharide Compositional Analysis of Heparin and Low Molecular Weight Heparins by Capillary Electrophoresis
(1993)The application of capillary electrophoresis to total compositional analysis of heparin and low-molecular-weight heparin samples has been studied. Optimum resolution of 17 defined oligosaccharides was obtained with the ... -
Oligosaccharide Mapping of Low Molecular Weight Heparins: Structural Differences and their Relationship to Activity
(1990)Low molecular weight heparins from a variety of commercial sources were examined. These had been prepared by several methods including peroxidative cleavage, nitrous acid cleavage, chemical beta-elimination, enzymatic ... -
On-line CE-LIF-MS analysis of glycans labeled with teal fluorescence dye using electrokinetic sheath liquid pump-based nanospray ion source
(2018)Rationale: N-linked glycan analysis of recombinant therapeutic proteins, such as monoclonal antibodies, Fc-fusion proteins, and antibody-drug conjugates, provides valuable information regarding protein therapeutics ... -
On-line separation and characterization of hyaluronan oligosaccharides derived from radical depolymerization
(Elsevier, 2013-01-01)Hydroxyl radicals are widely implicated in the oxidation of carbohydrates in biological and industrial processes and are often responsible for their structural modification resulting in functional damage. In this study, ... -
One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan
(2022-01-01)Heparin, a glycosaminoglycan-based anticoagulant drug, is prepared as an extract of animal tissues. Heparosan, an Escherichia coli (E. coli) K5 capsular polysaccharide with the structure →4)-β-D-glucuronic acid (1 → ... -
Online capillary zone electrophoresis negative electron transfer dissociation tandem mass spectrometry of glycosaminoglycan mixtures
(Elsevier, 2019-11-01)Glycosaminoglycans (GAGs) are important biological molecules that are highly anionic and occur in nature as complex mixtures. A platform that combines capillary zone electrophoresis (CZE) separations with mass spectrometry ... -
Optimization of bioprocess conditions improves production of a CHO cell-derived, bioengineered heparin
(Wiley, 2015-07-01)Heparin is the most widely used anticoagulant drug in the world today. Heparin is currently produced from animal tissues, primarily porcine intestines. A recent contamination crisis motivated development of a non-animal-derived ...