Browsing Linhardt Research Labs Papers by Title
Now showing items 991-1010 of 1022
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Thin Layer Chromatography for the Separation and Analysis of Acidic Carbohydrates
(2009-10-13)Thin layer chromatography (TLC) is routinely used by synthetic organic chemists and natural products chemists working on a variety of types of molecules. Carbohydrate chemists and biochemists in the past largely relied on ... -
Three Dimensional Cellular Microarray Platform for Human Neural Stem Cell Differentiation and Toxicology
(Elsevier, 2014-01-01)We developed a three-dimensional (3D) cellular microarray platform for the high-throughput (HT) analysis of human neural stem cell (hNSC) growth and differentiation. The growth of an immortalized hNSC line, ReNcell VM, was ... -
Tip-enhanced Raman imaging of single-stranded DNA with single base resolution
(2019-01-16)Tip-enhanced Raman scattering (TERS) is a promising optical and analytical technique for chemical imaging and sensing at single molecule resolution. In particular, TERS signals generated by a gap-mode configuration where ... -
Tissue Distribution [14 C] Sucrose Octasulfate Following Oral Administration to Rats
(2002)Purpose: Aluminum sucrose octasulfate (SOS) is used clinically to prevent ulcers. Under physiologic conditions, the sodium salt of this drug can be formed. Our objective was to determine whether sodium SOS was absorbed ... -
Tissue Distribution and Antithrombotic Activity of Unlabeled or 14C-Labelled Porcine Intestinal Mucosal Heparin Following Administration to Rats by the Oral Route
(2000-01-01)Distribution and antithrombotic activity of orally administered unfractionated porcine heparin were studied. [14C]Heparin was prepared by de-N-acetylation of porcine mucosal heparin followed by re-N-acetylation, using ... -
Toll-like Receptor 4 Mediates Induction of Inflammatory Pathway by Food Additive Carrageenan
(2008)The sulfated polysaccharide carrageenan (CGN) induces activation of NFκB and interleukin 8 (IL-8) in human colonic epithelial cells through a pathway of innate immunity mediated by Bcl10 (B-cell CLL/lymphoma 10). In this ... -
Top-down approach for the direct characterization of low molecular weight heparins using LC-FT-MS
(American Chemical Society (ACS), 2012-10-16)Low molecular heparins (LMWHs) are structurally complex, heterogeneous, polydisperse, and highly negatively charged mixtures of polysaccharides. The direct characterization of LMWH is a major challenge for currently available ... -
Total Synthesis of Quercetin 3-Sophorotrioside
(2004-03-19)5,7-dihydroxy-3-[beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl]-2-(3,4-dihydroxyphenyl)-4H-1-benzopyran-4-one (quercetin 3-sophorotrioside), a flavonol triglycoside, isolated from Pisum ... -
Toward the chemoenzymatic synthesis of heparan sulfate oligosaccharides: Oxidative cleavage of p-nitrophenyl group with ceric ammonium salts
(Elsevier, 2013-08-14)We have developed an efficient chemoenzymatic synthesis of heparan sulfate oligosaccharides employing the para-nitrophenyl (p-NP) β-glucuronide as an acceptor compatible with enzymatic elongation and one that significantly ... -
Trifluoroethylsulfonate Protected Monosaccharides in Glycosylation Reactions
(2004-08-16)A variety of sulfo-protected monosaccharide donors and acceptors were investigated in glycosylation reactions. Trifluoroethylsulfonate (SO3TFE) group was compatible with a wide range of activation conditions commonly used ... -
Trimer hydroxylated quinone (IIIHyQ) derived from apocynin targets cysteine residues of p47phox preventing the activation of human vascular NADPH oxidase
(Elsevier, 2012)Enzymatically derived oligophenols from apocynin can be effective inhibitors of human vascular NADPH oxidase (Nox). An isolated trimer hydroxylated quinone (IIIHyQ) has been shown to inhibit endothelial NADPH oxidase with ... -
Tunable thermo-responsive poly(N-vinylcaprolactam) cellulose nanofibers: Synthesis, characterization, and fabrication of potential affinity membranes
(2013)Temperature-responsive PVCL homopolymers and functional PVCL polymers containing carboxylic acids are prepared in organic and aqueous solutions. PVCL bulk polymers are characterized using 1H NMR, photometry, ATR-FTIR, and ... -
Turkey Intestines as a Commercial Source of Heparin? Comparative Structural Studeis of Intestinal Avian and Mammalian Glycosaminoglycans
(2003)Heparin is a glycosaminoglycan (GAG) that is extracted primarily from porcine intestinal tissues and is widely used as a clinical anticoagulant. It is biosynthesized as a proteoglycan and stored exclusively in mast cells ... -
Two-Dimension Affinity Resolution Electrophoresis Demonstrates that Three Distinct Heparin Populations Interact with Antithrombin III
(1995)Tween 20 is frequently used in blotting analysis of proteins and nucleic acids to decrease background staining due to nonspecific binding of antibodies to various membranes. Inclusion of 0.1% (w/v) Tween 20 in the washing ... -
Ultra-High Resolution Separation Comes of Age
(2002-11-15)Born in the late 1960s (1), popularized in its infancy for its high resolution (2), and reaching puberty in the mid 1980s with the introduction of commercial instrumentation (3), capillary electrophoresis (CE) has finally ... -
Ultraperformance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis
(Elsevier, 2011)A high resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultraperformance liquid chromatography in a reverse-phase, ion-pairing ... -
Ultraperformance liquid chromatography with electrospray ion trap mass spectrometry for chondroitin disaccharide analysis
(Elsevier, 2010-02-01)Chondroitin sulfate (CS) has an important role in cell division, in the central nervous system, and in joint-related pathologies such as osteoarthritis. Due to the complex chemical structure and biological importance of ... -
Ultrasensitive detection and quantification of acidic disaccharides using capillary electrophoresis and quantum dot-based fluorescence resonance energy transfer
(American Chemical Society (ACS), 2013-10-01)Rapid and highly sensitive detection of the carbohydrate components of glycoconjugates is critical for advancing glycobiology. Fluorescence (or Förster) resonance energy transfer (FRET) is commonly used in detection of ... -
Ultrasound-assisted fast preparation of low molecular weight fucosylated chondroitin sulfate with antitumor activity
(2019-04-01)Fucosylated chondroitin sulfate from sea cucumber Isostichopus badionotus (fCS-Ib) was depolymerized with an ultrasound-accelerated, metal-free Fenton chemistry, relying on H2O2/ascorbic acid redox system. Fragments of ... -
Uniform nanoparticle coating of cellulose fibers during wet electrospinning
(2014-09-28)This work outlines a newly developed method that allows electrospun cellulose fibers to be coated with nanoparticles during dry-jet wet electrospinning. Cellulose fibers were wet electrospun from a room temperature ionic ...