Browsing Linhardt Research Labs Papers by Title
Now showing items 41-60 of 1022
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Affinity Purification of Secreted Alkaline Phosphatase Produced by the Baculovirus Expression Vector System
(2001-01-01)Human secreted alkaline phosphatase (SEAP) was produced in a stably-transformed Spodoptera frugiperda Sf-9 insect cell line (Sfb4GalT) following infection with a recombinant Autographa californica multiple nuclear ... -
Affinity, kinetic and structural study of the interaction of 3-O-sulfotransferase isoform 1 with heparan sulfate
(American Chemical Society (ACS), 2006-04-25)The 3-O-sulfonation of glucosamine residues in heparan sulfate (HS) by 3-O-sulfotransferase (3-OST) is a key substitution that is present in HS sequences of biological importance, in particular HS anticoagulant activity. ... -
Akebia saponin D reverses corticosterone hypersecretion in an Alzheimer's disease rat model
(2018-11-01)Background: Glucocorticoid hormones are implicated in the pathogenesis of Alzheimer's disease (AD) and other diseases including diabetes, hyperlipidemia, and osteoporosis. Akebia saponin D (ASD) possesses numerous ... -
Alveolar heparan sulfate shedding impedes recovery from bleomycin-induced lung injury
(2020-06-01)The pulmonary epithelial glycocalyx, an anionic cell surface layer enriched in glycosaminoglycans such as heparan sulfate and chondroitin sulfate, contributes to the alveolar barrier. Direct injury to the pulmonary epithelium ... -
Amphiphilic bromelain-synthesized oligo-phenylalanine grafted with methoxypolyethylene glycol possessing stabilizing thermo-responsive emulsion properties
(2019-03-07)A thermo-responsive amphiphile was developed from oligo-phenylalanine [oligo(Phe)]. The hydrophobic moiety of the amphiphile, oligo(Phe) was synthesized via reverse hydrolysis catalyzed by bromelain in dimethyl sulfoxide ... -
Amphiphilic mPEG-modified oligo-phenylalanine nanoparticles chemoenzymatically synthesized via papain
(American Chemical Society (ACS), 2020-11-24)Amphiphilic mPEG-modified peptide nanoparticles were developed from oligo-phenylalanine (OPhe) nanoparticles (NPs) synthesized via papain. Tyndall effects indicate that OPhe NPs are amphiphobic. Addition of protein ... -
An Electrophoresis-Based Assay For Glycosyltransferase Activity
(1992-08-15)Polyacrylamide gel electrophoresis (PAGE) and capillary zone electrophoresis (CZE) were used to measure the activity of glycosyltransferases. Acceptor molecules were prepared by reductive amination of the monopotassium ... -
An Enzymatic System for Removing Heparin in Extracorporeal Therapy
(1982-01-01)The need to fully heparinize patients undergoing extracorporeal therapy often leads to hemorrhagic complications. To enable heparinization of only the extracorporeal circuit, a blood filter containing immobilized heparinase ... -
An Immobilized Microbial Heparinase for Blood Deheparinization
(1984-02-01)A new medical application of an immobilized microbial enzyme is described. Extracorporeal devices require systemic heparin administration to prevent thrombus formation; however, the use of heparin often leads to serious ... -
An ‘omics approach towards CHO cell engineering
(2013-05-01)Chinese hamster ovarian cells (CHO) cells have been extensively utilized for industrial production of biopharmaceutical products, such as monoclonal antibodies, human growth hormones, cytokines, and blood-products. Recent ... -
Analysis of 3-O-sulfo group containing heparin tetrasaccharides in heparin by liquid chromatography-mass spectrometry
(Elsevier, 2014-06-15)Complete heparin digestion with heparin lyase 2 affords a mixture of disaccharides and resistant tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends. Quantitative online liquid ... -
Analysis of E. coli K5 capsular polysaccharide heparosan
(Springer, 2011-01-01)Heparosan is the key precursor for the preparation of bioengineered heparin, a potential replacement for porcine intestinal heparin, an important anticoagulant drug. The molecular weight (MW) distribution of heparosan ... -
Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry
(1999-01-01)Neutral oligosaccharides were fluorescently conjugated with 7-amino-1, 3-naphthalenedisulfonic acid. A mixture of fluorescently labeled chitobiose, chitotriose, and chitotetrose were successfully separated by preparative ... -
Analysis of Fluorescently Labeled Sugars by Reversed-Phase Ion-Pairing High Performance Liquid Chromatography
(1995-01-01)Reducing sugars, including monosaccharides, disaccharides, and a trisaccharide, are derivatized by reductive amination with 7-amino-1,3-naphthalene disulfonic acid. Reversed-phase ion-pairing high-performance liquid ... -
Analysis of glycosaminoglycan-derived disaccharides by capillary electrophoresis using laser-induced fluorescence detection
(Elsevier, 2012-08-01)A quantitative and highly sensitive method for the analysis of glycosaminoglycan (GAG)-derived disaccharides is presented that relies on capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection. This ... -
Analysis of Glycosaminoglycan-Derived Oligosaccharides Using Fast Atom Bombardment Mass Spectrometry
(1992-02-17)Glycosaminoglycans (GAGS) are sulfated, linear polysaccharides found in mammalian tissues’, that play an important role in cell-cell interaction2 and demonstrate a wide variety of other important biological activities3. ... -
Analysis of Glycosaminoglycan-Derived Oligosaccharides Using Reversed Phase Ion-Pairing HPLC and Ion Exchange Chromatography With Suppressed Conductivity Detection
(1989)Oligosaccharides prepared from glycosaminoglycans (GAGs) including heparin, heparan sulfate, chondroitin sulfates, dermatan sulfate, and keratan sulfate were analyzed using reverse-phase ion-pairing HPLC and ion-exchange ... -
Analysis of glycosaminoglycans in stem cell glycomics
(Springer, 2011-12-01)Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, ... -
Analysis of Glycosaminoglycans with Polysaccharide Lyases
(2001-01-01)Polysaccharide lyases are a class of enzymes useful for analysis of glycosaminoglycans (GAGs) and the glycosaminoglycan component of proteoglycans (PGs). These enzymes cleave specific glycosidic linkages present in acidic ... -
Analysis of heparin oligosaccharides by capillary electrophoresis- negative-ion electrospray ionization mass spectrometry
(Springer, 2017-01-01)Most hyphenated analytical approaches that rely on liquid chromatography–MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with ...