Browsing Linhardt Research Labs Papers by Author "Cai, Chao"
Now showing items 1-17 of 17
-
A novel structural fucosylated chondroitin sulfate from Holothuria mexicana and its effects on growth factors binding and anticoagulation
Li, Qinying; Cai, Chao; Chang, Yaoguang; Zhang, Fuming; Linhardt, Robert J.; Xue, Changhu; Li, Guoyun; Yu, Guangli (2018-02-01)Fucosylated chondroitin sulfate (FCS), a structurally distinct glycosaminoglycan from the body wall of sea cucumber, possesses many biological properties and pharmacology functions. The refined structure of FCS isolated ... -
A purification process for heparin and precursor polysaccharides using the pH responsive behavior of chitosan
Bhaskar, Ujjwal; Hickey, Anne M.; Li, Guoyun; Mundra, Ruchir V.; Zhang, Fuming; Fu, Li; Cai, Chao; Ou, Zhimin; Dordick, Jonathan S.; Linhardt, Robert J. (Wiley, 2015-09-01)The contamination crisis of 2008 has brought to light several risks associated with use of animal tissue derived heparin. Because the total chemical synthesis of heparin is not feasible, a bioengineered approach has been ... -
Capillary electrophoresis for total glycosaminoglycan analysis
Ucakturk, Ebru; Cai, Chao; Li, Lingyun; Li, Guoyun; Zhang, Fuming; Linhardt, Robert J. (2014-01-01)A capillary zone electrophoresis-laser induced fluorescence detection (CZE-LIF) method was developed for the simultaneous analysis of disaccharides derived from heparan sulfate, chondroitin sulfate/dermatan sulfate, ... -
Click-coated, heparinized, decellularized vascular grafts
Dimitrievska, Sashka; Cai, Chao; Weyers, Amanda; Balestrini, Jenna L.; Lin, Tylee; Sundaram, Sumati; Hatachi, Go; Spiegel, David A.; Kyriakides, Themis R.; Miao, Jianjun; Li, Guoyun; Niklason, Laura E.; Linhardt, Robert J. (Elsevier, 2015-02-01)A novel method enabling the engineering of a dense and appropriately oriented heparin-containing layer on decellularized aortas has been developed. Amino groups of decellularized aortas were first modified to azido groups ... -
End-functionalised glycopolymers as glycosaminoglycan mimetics inhibit HeLa cell proliferation
Yang, Chendong; Gao, Lei; Shao, Meng; Cai, Chao; Wang, Lihao; Chen, Yifan; Li, Jianghua; Fan, Fei; Han, Yubing; Liu, Ming; Linhardt, Robert J.; Yu, Guangli (2020-08-07)The glycosaminoglycans (GAGs) on cell surfaces play significant roles during cancer development, and the heparanase activity is strongly implicated in the structural remodeling of the extracellular GAG matrix, potentially ... -
Fluorous-assisted chemoenzymatic synthesis of heparan sulfate oligosaccharides
Cai, Chao; Dickinson, Demetria M.; Li, Lingyun; Masuko, Sayaka; Suflita, Matt; Schultz, Victor; Nelson, Shawn D.; Bhaskar, Ujjwal; Liu, Jian; Linhardt, Robert J. (American Chemical Society (ACS), 2014-04-18)Fluorous-assisted chemoenzymatic synthesis of heparan sulfate oligosaccharides -
Glycocalyx-like hydrogel coatings for small diameter vascular grafts
Dimitrievska, Sashka; Wang, Juan; Lin, Tylee; Weyers, Amanda; Bai, Hualong; Qin, Lingfeng; Li, Guangxin; Cai, Chao; Kypson, Alan; Kristofik, Nina; Gard, Ashley; Sundaram, Sumati; Yamamoto, Kota; Wu, Wei; Zhao, Liping; Kural, Mehmet H.; Yuan, Yifan; Madri, Joseph; Kyriakides, Themis R.; Linhardt, Robert J.; Niklason, Laura E. (2020-06-01)Novel biological vascular conduits, such as decellularized tissue engineered vascular grafts (TEVGs) are hindered by high thrombogenicity. To mimic the antithrombogenic surface of native vessels with a continuous ... -
Heavy heparin: A stable isotope-enriched, chemoenzymatically-synthesized, poly-component drug
Cress, Brady F.; Bhaskar, Ujjwal; Vaidyanathan, Deepika; Williams, Asher; Cai, Chao; Liu, Xinyue; Fu, Li; M-Chari, Vandhana; Zhang, Fuming; Mousa, Shaker A.; Dordick, Jonathan S.; Koffas, Mattheos A.G.; Linhardt, Robert J. (Wiley, 2019-04-23)Heparin is a highly sulfated, complex polysaccharide and widely used anticoagulant pharmaceutical. In this work, we chemoenzymatically synthesized perdeuteroheparin from biosynthetically enriched heparosan precursor obtained ... -
Interaction of Neisseria meningitidis group X N-acetylglucosamine-1-phosphotransferase with its donor substrate
Ming, Shonoi A.; Cottman-Thomas, Ebony; Black, Natalee C.; Chen, Yi; Veeramachineni, Vamsee; Peterson, Dwight C.; Chen, Xi; Tedaldi, Lauren M.; Wagner, Gerd K.; Cai, Chao; Linhardt, Robert J.; Vann, Willie F. (Oxford University Press, 2018-02-01)Neisseria meningitidis Group X is an emerging cause of bacterial meningitis in Sub-Saharan Africa. The capsular polysaccharide of Group X is a homopolymer of N-acetylglucosamine α(1–4) phosphate and is a vaccine target for ... -
Keratan sulfate glycosaminoglycan from chicken egg white
Fu, Li; Sun, Xiaojun; He, Wenqin; Cai, Chao; Onishi, Akihiro; Zhang, Fuming; Linhardt, Robert J.; Liu, Zhangguo (2016-07-01)Keratan sulfate (KS) was isolated from chicken egg white in amounts corresponding to ∼0.06 wt% (dry weight). This KS had a weight-average molecular weight of ∼36–41 kDa with a polydispersity of ∼1.3. The primary repeating ... -
Mass spectrometric evidence for the mechanism of free-radical depolymerization of various types of glycosaminoglycans
Zhang, Ning; Li, Guoyun; Li, Shijie; Cai, Chao; Zhang, Fuming; Linhardt, Robert J.; Yu, Guangli (2020-04-01)Glycosaminoglycans (GAGs) are large, complex carbohydrate molecules that interact with a wide range of proteins involved in physiological and pathological processes. Several naturally derived GAGs have emerged as potentially ... -
Preparation and application of a “clickable” acceptor for enzymatic synthesis of heparin oligosaccharides
Cai, Chao; Edgar, Kristi; Liu, Jian; Linhardt, Robert J. (Elsevier, 2013-05-03)A 'clickable' disaccharide was prepared by treating the aldehyde precursor with hydroxylamine, followed by the catalytic hydrogenation and diazotransfer reaction. This disaccharide was successfully applied to the elongation ... -
Recent Progress and Advanced Technology in Carbohydrate-Based Drug Development
Pan, Lin; Cai, Chao; Liu, Chanjuan; Liu, Di; Li, Guoyun; Linhardt, Robert J.; Yu, Guangli (2021-06-01)Carbohydrates, one of the most abundant and widespread biomolecules in nature, play indispensable roles in diverse biological functions, and represent a treasure trove of untapped potential for pharmaceutical applications. ... -
Semi-synthesis of chondroitin sulfate-E from chondroitin sulfate-A
Cai, Chao; Solakyildirim, Kemal; Yang, Bo; Beaudet, Julie M.; Weyers, Amanda; Linhardt, Robert J.; Zhang, Fuming (Elsevier, 2012-01-04)Chondroitin sulfate-E (chondroitin-4, 6-disulfate) was prepared from chondroitin sulfate-A (chondroitin-4 - sulfate) by regioselective sulfonation, performed using trimethylamine sulfur trioxide in formamide under argon. ... -
Structural insight into the binding of human galectins to corneal keratan sulfate, its desulfated form and related saccharides
Miller, Michelle C.; Cai, Chao; Wichapong, Kanin; Bhaduri, Sayantan; Pohl, Nicola L.B.; Linhardt, Robert J.; Gabius, Hans Joachim; Mayo, Kevin H. (Nature, 2020-12-01)Glycosaminoglycan chains of keratan sulfate proteoglycans appear to be physiologically significant by pairing with tissue lectins. Here, we used NMR spectroscopy and molecular dynamics (MD) simulations to characterize ... -
Toward the chemoenzymatic synthesis of heparan sulfate oligosaccharides: Oxidative cleavage of p-nitrophenyl group with ceric ammonium salts
Cai, Chao; Li, Lingyun; Harvey, Cate; Liu, Jian; Linhardt, Robert J. (Elsevier, 2013-08-14)We have developed an efficient chemoenzymatic synthesis of heparan sulfate oligosaccharides employing the para-nitrophenyl (p-NP) β-glucuronide as an acceptor compatible with enzymatic elongation and one that significantly ... -
Ultrasensitive detection and quantification of acidic disaccharides using capillary electrophoresis and quantum dot-based fluorescence resonance energy transfer
Chang, Yuqing; Cai, Chao; Li, Lingyun; Miao, Jianjun; Ucakturk, Ebru; Li, Guoyun; Ly, Mellisa; Linhardt, Robert J. (American Chemical Society (ACS), 2013-10-01)Rapid and highly sensitive detection of the carbohydrate components of glycoconjugates is critical for advancing glycobiology. Fluorescence (or Förster) resonance energy transfer (FRET) is commonly used in detection of ...