Now showing items 1-16 of 16

    • An ‘omics approach towards CHO cell engineering 

      Datta, Payel; Linhardt, Robert J.; Sharfstein, Susan T. (2013-05-01)
      Chinese hamster ovarian cells (CHO) cells have been extensively utilized for industrial production of biopharmaceutical products, such as monoclonal antibodies, human growth hormones, cytokines, and blood-products. Recent ...
    • Anti-SARS-CoV-2 activity of rhamnan sulfate from Monostroma nitidum 

      Song, Yuefan; He, Peng; Rodrigues, Andre L.; Datta, Payel; Tandon, Ritesh; Bates, John T.; Bierdeman, Michael A.; Chen, Chen; Dordick, Jonathan; Zhang, Fuming; Linhardt, Robert J. (Multidisciplinary Digital Publishing Institute (MDPI), 2021-12-01)
      The COVID-19 pandemic is a major human health concern. The pathogen responsible for COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), invades its host through the interaction of its spike (S) protein ...
    • Bioengineered Chinese hamster ovary cells with Golgi-targeted 3-O-sulfotransferase-1 biosynthesize heparan sulfate with an antithrombin binding site 

      Datta, Payel; Li, Guoyun; Yang, Bo; Zhao, Xue; Baik, Jong Youn; Gemmill, Trent R.; Sharfstein, Susan T.; Linhardt, Robert J. (The American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2013-12-27)
      HS3st1 (heparan sulfate 3-O-sulfotransferase isoform-1) is a critical enzyme involved in the biosynthesis of the antithrombin III (AT)-binding site in the biopharmaceutical drug heparin. Heparin is a highly sulfated ...
    • Bioengineering murine mastocytoma cells to produce anticoagulant heparin 

      Gasimli, Leyla; Glass, Charles A.; Datta, Payel; Yang, Bo; Li, Guoyun; Gemmill, Trent R.; Baik, Jong Youn; Sharfstein, Susan T.; Esko, Jeffrey D.; Linhardt, Robert J. (2014-03-01)
      Heparin (HP), an important anticoagulant polysaccharide, is produced in a complex biosynthetic pathway in connective tissue-type mast cells. Both the structure and size of HP are critical factors determining the anticoagulation ...
    • Cell-based microscale isolation of glycoaminoglycans for glycomics study 

      Zhao, Xue; Yang, Bo; Datta, Payel; Gasmili, Leyla; Zhang, Fuming; Linhardt, Robert J. (Taylor and Francis, 2012-07-09)
      Glycomics research requires the isolation of glycans from cells for structural characterization and functional studies of the glycans. A method for cell-based microscale isolation and quantification of highly sulfated, ...
    • Complete biosynthesis of a sulfated chondroitin in Escherichia coli 

      Badri, Abinaya; Williams, Asher; Awofiranye, Adeola; Datta, Payel; Xia, Ke; He, Wenqin; Fraser, Keith; Dordick, Jonathan S.; Linhardt, Robert J.; Koffas, Mattheos A.G. (Nature, 2021-12-01)
      Sulfated glycosaminoglycans (GAGs) are a class of important biologics that are currently manufactured by extraction from animal tissues. Although such methods are unsustainable and prone to contamination, animal-free ...
    • Enzymatic generation of highly anticoagulant bovine intestinal heparin 

      Fu, Li; Li, Kevin; Mori, Daisuke; Hirakane, Makoto; Lin, Lei; Grover, Navdeep; Datta, Payel; Yu, Yanlei; Zhao, Jing; Zhang, Fuming; Yalcin, Murat; Mousa, Shaker A.; Dordick, Jonathan S.; Linhardt, Robert J. (2017-10-26)
      Unlike USP porcine heparin, bovine intestinal heparin (BIH) has a low anticoagulant activity. Treatment with 6-OST-1, -3, and/or 3-OST-1 afforded two remodeled heparins that met USP heparin activity and Mw specifications. ...
    • Evaluating heparin products for heparin-induced thrombocytopenia using surface plasmon resonance 

      Zhang, Fuming; Datta, Payel; Dordick, Jonathan S.; Linhardt, Robert J. (2020-02-01)
      Heparin-induced thrombocytopenia (HIT) is an adverse immunological disorder caused by antibodies to platelet factor 4 (PF4)-heparin complexes. The analysis of HIT potential for different heparin and heparin-related products ...
    • Heparosan Chain Characterization: Sequential Depolymerization of E. coli K5 Heparosan by a Bacterial Eliminase Heparin Lyase III and a Bacterial Hydrolase Heparanase Bp to Prepare Defined Oligomers, 

      Datta, Payel; Yan, Lu Feng; Awofiranye, Adeola; Dordick, Jonathan S.; Linhardt, Robert J. (2021-03-01)
      Heparosan is a non-sulfated polysaccharide and potential applications include, chemoenzymatic synthesis of heparin and heparan sulfates. Heparosan is produced using microbial cells (natural producers or engineered cells). ...
    • Industrial production of glycosaminoglycans 

      Datta, Payel; Linhardt, Robert J.; Sharfstein, Susan T. (2019-01-01)
      Glycosaminoglycans (GAGs) are naturally occurring polysaccharides, composed of alternating sugar units; these include an amino sugar (e.g., N-acetyl glucosamine, GlcNAc or N-acetyl galactosamine, GalNAc) and either a ...
    • Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin 

      Baik, Jong Youn; Gasimli, Leyla; Yang, Bo; Datta, Payel; Zhang, Fuming; Glass, Charles A.; Esko, Jeffrey D.; Linhardt, Robert J.; Sharfstein, Susan T. (Elsevier, 2012-03-01)
      Heparin is the most widely used pharmaceutical to control blood coagulation in modern medicine. A health crisis that took place in 2008 led to a demand for production of heparin from non-animal sources. Chinese hamster ...
    • Microarray platform affords improved product analysis in mammalian cell growth studies 

      Datta, Payel; Meli, Luciana; Li, Lingyun; Migliore, Nicole; Schaefer, Eugene; Sharfstein, Susan T.; Dordick, Jonathan S.; Linhardt, Robert J. (Wiley, 2014-01-01)
      High throughput (HT) platforms serve as a cost-efficient and rapid screening method for evaluating the effect of cell-culture conditions and screening of chemicals. We report the development of a HT cell-based microarray ...
    • Microscale separation of heparosan, heparan sulfate and heparin 

      Zhao, Xue; Yang, Bo; Linkens, Kathryn; Datta, Payel; Onishi, Akihiro; Zhang, Fuming; Linhardt, Robert J. (Elsevier, 2013-03-15)
      The separation and quantification of glycosaminoglycan (GAG) chains, with different levels of sulfation, from cells, media and prepared through chemoenzymatic synthesis or metabolic engineering, poses a major challenge in ...
    • Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells 

      Datta, Payel; Yang, Bo; Linhardt, Robert J.; Sharfstein, Susan T. (2015-03-18)
      Sodium butyrate, a histone deacetylase inhibitor, has been used to improve transgene expression in Chinese hamster ovary (CHO) cells. The current study explores the impact of butyrate treatment on heparan sulfate (HS) ...
    • Optimization of bioprocess conditions improves production of a CHO cell-derived, bioengineered heparin 

      Baik, Jong Youn; Dahodwala, Hussain; Oduah, Eziafa; Talman, Lee; Gemmill, Trent R.; Gasimli, Leyla; Datta, Payel; Yang, Bo; Li, Guoyun; Zhang, Fuming; Li, Lingyun; Linhardt, Robert J.; Campbell, Andrew M.; Gorfien, Stephen F.; Sharfstein, Susan T. (Wiley, 2015-07-01)
      Heparin is the most widely used anticoagulant drug in the world today. Heparin is currently produced from animal tissues, primarily porcine intestines. A recent contamination crisis motivated development of a non-animal-derived ...
    • Platelet factor 4 polyanion immune complexes: Heparin induced thrombocytopenia and vaccine-induced immune thrombotic thrombocytopenia 

      Datta, Payel; Zhang, Fuming; Dordick, Jonathan S.; Linhardt, Robert J. (Springer, 2021-12-01)
      Background: This is a review article on heparin-induced thrombocytopenia, an adverse effect of heparin therapy, and vaccine-induced immune thrombotic thrombocytopenia, occurring in some patients administered certain ...