Browsing Linhardt Research Labs Papers by Author "Han, Xiaorui"

Now showing items 1-20 of 21

    • A rolling circle amplification based platform for ultrasensitive detection of heparin 

      Lin, Lei; Li, Bingzhi; Han, Xiaorui; Zhang, Fuming; Zhang, Xing; Linhardt, Robert J. (2021-01-21)
      Heparin has a variety of pharmacological uses, including applications for anti-tumor metastasis, anti-inflammatory and anti-viral activities and is widely used as a clinical anticoagulant. Due to its widespread applications ...

    • Autophagic degradation of HAS2 in endothelial cells: A novel mechanism to regulate angiogenesis 

      Chen, Carolyn G.; Gubbiotti, Maria A.; Kapoor, Aastha; Han, Xiaorui; Yu, Yanglei; Linhardt, Robert J.; Iozzo, Renato V. (2020-08-01)
      Hyaluronan plays a key role in regulating inflammation and tumor angiogenesis. Of the three transmembrane hyaluronan synthases, HAS2 is the main pro-angiogenic enzyme responsible for excessive hyaluronan production. We ...

    • Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats 

      Zhang, Xiaolu; Hashimoto, Joel G.; Han, Xiaorui; Zhang, Fuming; Linhardt, Robert J.; Guizzetti, Marina (Springer, 2021-03-01)
      Astrocytes are major producers of the extracellular matrix (ECM), which is involved in the plasticity of the developing brain. In utero alcohol exposure alters neuronal plasticity. Glycosaminoglycans (GAGs) are a family ...

    • Chemometric analysis of porcine, bovine and ovine heparins 

      Ouyang, Yilan; Han, Xiaorui; Yu, Yanlei; Chen, Jianle; Fu, Li; Zhang, Fuming; Linhardt, Robert J.; Fareed, Jawed; Hoppensteadt, Debra; Jeske, Walter; Kouta, Ahmed; Zhang, Zhenqing; Xia, Ke (2019-02-05)
      Heparin is a polysaccharide anticoagulant drug isolated from animal tissues. There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption. ...

    • Circulating heparan sulfate fragments mediate septic cognitive dysfunction 

      Hippensteel, Joseph A.; Anderson, Brian J.; Orfila, James E.; McMurtry, Sarah A.; Dietz, Robert M.; Su, Guowei; Ford, Joshay A.; Oshima, Kaori; Yang, Yimu; Zhang, Fuming; Han, Xiaorui; Yu, Yanlei; Liu, Jian; Linhardt, Robert J.; Meyer, Nuala J.; Herson, Paco S.; Schmidt, Eric P. (2019-01-01)
      Septic patients frequently develop cognitive impairment that persists beyond hospital discharge. The impact of sepsis on electrophysiological and molecular determinants of learning is underexplored. We observed that mice ...

    • Circulating heparin oligosaccharides rapidly target the hippocampus in sepsis potentially impacting cognitive functions 

      Zhang, Xing; Han, Xiaorui; Xia, Ke; Xu, Yongmei; Yang, Yimu; Oshima, Kaori; Haeger, Sarah M.; Perez, Mario J.; McMurtry, Sarah A.; Hippensteel, Joseph A.; Ford, Joshay A.; Herson, Paco S.; Liu, Jian; Schmidt, Eric P.; Linhardt, Robert J. (2019-05-07)
      Sepsis induces heparanase-mediated degradation of the endothelial glycocalyx, a heparan sulfate-enriched endovascular layer critical to vascular homeostasis, releasing highly sulfated domains of heparan sulfate into the ...

    • Design of anti-inflammatory heparan sulfate to protect against acetaminophen-induced acute liver failure 

      Arnold, Katelyn; Xu, Yongmei; Sparkenbaugh, Erica M.; Li, Miaomiao; Han, Xiaorui; Zhang, Xing; Xia, Ke; Piegore, Mark; Zhang, Fuming; Zhang, Xiaoxiao; Henderson, Mike; Pagadala, Vijayakanth; Su, Guowei; Tan, Lisi; Park, Pyong Woo; Stravitz, Richard T.; Key, Nigel S.; Linhardt, Robert J.; Pawlinski, Rafal; Xu, Ding; Liu, Jian (American Association for the Advancement of Science (AAAS), 2020-03-18)
      Acetaminophen/paracetamol (APAP) overdose is the leading cause of drug-induced acute liver failure (ALF) in the United States and Europe. The progression of the disease is attributed to sterile inflammation induced by the ...

    • Detection of glycosaminoglycans by polyacrylamide gel electrophoresis and silver staining 

      Lariviere, Wells B.; Han, Xiaorui; Oshima, Kaori; McMurtry, Sarah A.; Linhardt, Robert J.; Schmidt, Eric P. (Jove, 2021-02-01)
      Sulfated glycosaminoglycans (GAGs) such as heparan sulfate (HS) and chondroitin sulfate (CS) are ubiquitous in living organisms and play a critical role in a variety of basic biological structures and processes. As polymers, ...

    • Epithelial Heparan Sulfate Contributes to Alveolar Barrier Function and is Shed during Lung Injury 

      Haeger, Sarah M.; Liu, Xinyue; Han, Xiaorui; Brennan McNeil, J.; Oshima, Kaori; McMurtry, Sarah A.; Yang, Yimu; Ouyang, Yilan; Zhang, Fuming; Nozik-Grayck, Eva; Zemans, Rachel L.; Tuder, Rubin M.; Bastarache, Julie A.; Linhardt, Robert J.; Schmidt, Eric P. (2018-09-01)
      The lung epithelial glycocalyx is a carbohydrate-enriched layer lining the pulmonary epithelial surface. Although epithelial glycocalyx visualization has been reported, its composition and function remain unknown. Using ...

    • Fibroblast growth factor signaling mediates pulmonary endothelial glycocalyx reconstitution 

      Yang, Yimu; Haeger, Sarah M.; Suflita, Matthew A.; Zhang, Fuming; Dailey, Kyrie L.; Colbert, James F.; Ford, Joshay A.; Picon, Mario A.; Stearman, Robert S.; Lin, Lei; Liu, Xinyue; Han, Xiaorui; Linhardt, Robert J.; Schmidt, Eric P. (2017-06-01)
      The endothelial glycocalyx is a heparan sulfate (HS)-rich endovascular structure critical to endothelial function. Accordingly, endothelial glycocalyx degradation during sepsis contributes to tissue edema and organ injury. ...

    • Functional role of glycosaminoglycans in decellularized lung extracellular matrix 

      Uhl, Franziska E.; Zhang, Fuming; Pouliot, Robert A.; Uriarte, Juan J.; Rolandsson Enes, Sara; Han, Xiaorui; Ouyang, Yilan; Xia, Ke; Westergren-Thorsson, Gunilla; Malmström, Anders; Hallgren, Oskar; Linhardt, Robert J.; Weiss, Daniel J. (Elsevier, 2020-01-15)
      Despite progress in use of decellularized lung scaffolds in ex vivo lung bioengineering schemes, including use of gels and other materials derived from the scaffolds, the detailed composition and functional role of ...

    • Intravenous fluid resuscitation is associated with septic endothelial glycocalyx degradation 

      Hippensteel, Joseph A.; Uchimido, Ryo; Tyler, Patrick D.; Burke, Ryan C.; Han, Xiaorui; Zhang, Fuming; McMurtry, Sarah A.; Colbert, James F.; Lindsell, Christopher J.; Angus, Derek C.; Kellum, John A.; Yealy, Donald M.; Linhardt, Robert J.; Shapiro, Nathan I.; Schmidt, Eric P. (Springer Nature, 2019-07-23)
      Background: Intravenous fluids, an essential component of sepsis resuscitation, may paradoxically worsen outcomes by exacerbating endothelial injury. Preclinical models suggest that fluid resuscitation degrades the endothelial ...

    • Loss of endothelial sulfatase-1 after experimental sepsis attenuates subsequent pulmonary inflammatory responses 

      Oshima, Kaori; Han, Xiaorui; Ouyang, Yilan; Masri, Rana El; Yang, Yimu; Haeger, Sarah M.; McMurtry, Sarah A.; Lane, Trevor C.; Davizon-Castillo, Pavel; Zhang, Fuming; Yue, Xinping; Vivès, Romain R.; Linhardt, Robert J.; Schmidt, Eric P. (2019-01-01)
      Sepsis patients are at increased risk for hospital-acquired pulmonary infections, potentially due to postseptic immunosuppression known as the compensatory anti-inflammatory response syndrome (CARS). CARS has been attributed ...

    • Metabolic engineering of cyanobacteria for photoautotrophic production of heparosan, a pharmaceutical precursor of heparin 

      Sarnaik, Aditya; Abernathy, Mary H.; Han, Xiaorui; Ouyang, Yilan; Xia, Ke; Chen, Yin; Cress, Brady; Zhang, Fuming; Lali, Arvind; Pandit, Reena; Linhardt, Robert J.; Tang, Yinjie J.; Koffas, Mattheos A.G. (2019-01-01)
      Heparosan is an unsulfated polysaccharide potentially important for its wide range of cosmetic and pharmaceutical applications, particularly as the precursor for the extensively used anticoagulant, heparin. Generally sourced ...

    • Negative-ion mode capillary isoelectric focusing-mass spectrometry for charge-based separation of acidic oligosaccharides 

      Ouyang, Yilan; Han, Xiaorui; Xia, Qiangwei; Chen, Jianle; Velagapudi, Sheila; Xia, Ke; Zhang, Zhenqing; Linhardt, Robert J. (2019-01-02)
      Glycosaminoglycans (GAGs) are biologically and pharmacologically important linear, anionic polysaccharides containing various repeating disaccharides sequences. The analysis of these polysaccharides generally relies on ...

    • Non-anticoagulant low molecular weight heparins for pharmaceutical applications 

      Ouyang, Yilan; Yu, Yanlei; Zhang, Fuming; Chen, Jianle; Han, Xiaorui; Xia, Ke; Yao, Yiming; Zhang, Zhenqing; Linhardt, Robert J. (2019-01-24)
      Heparin is a polypharmacological agent with anticoagulant activity. Periodate oxidation of the nonsulfated glucuronic acid residue results in non-anticoagulant heparin derivative (NACH) of reduced molecular weight. Similar ...

    • Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells toward Hepatocytes 

      Mikael, Paiyz E.; Willard, Charles; Koyee, Aurvan; Barlao, Charmaine Grace; Liu, Xinyue; Han, Xiaorui; Ouyang, Yilan; Xia, Ke; Linhardt, Robert J.; Dordick, Jonathan S. (2019-02-15)
      There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP). Currently, the vast ...

    • Structural analysis of urinary glycosaminoglycans from healthy human subjects 

      Han, Xiaorui; Sanderson, Patience; Nesheiwat, Sara; Lin, Lei; Yu, Yanlei; Zhang, Fuming; Amster, I. Jonathan; Linhardt, Robert J. (2020-01-01)
      Urinary glycosaminoglycans (GAGs) can reflect the health condition of a human being, and the GAGs composition can be directly related to various diseases. In order to effectively utilize such information, a detailed ...

    • Structural and functional components of the skate sensory organ ampullae of Lorenzini 

      Zhang, Xing; Xia, Ke; Lin, Lei; Zhang, Fuming; Yu, Yanlei; Ange, St Kalib; Han, Xiaorui; Edsinger, Eric; Sohn, Joel; Linhardt, Robert J. (2018-06-15)
      The skate, a cartilaginous fish related to sharks and rays, possesses a unique electrosensitive sensory organ known as the ampullae of Lorenzini (AoL). This organ is responsible for the detection of weak electric field ...

    • Synthesis and Characterization of Adipic Acid Derived Epoxy Resin 

      Maiorana, Anthony; Subramaniam, Bhagyashree; Centore, Robert; Han, Xiaorui; Linhardt, Robert J.; Gross, Richard A. (2016-08-15)
      Adipic acid, a highly abundant chemical that can be produced from biomass, was used to prepare an aromatic-free epoxy resin. Synthesis of the diglycidyl adipate was performed by a one-step process using epichlorohydrin and ...