Browsing Linhardt Research Labs Papers by Author "Nadkarni, V.D."
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Directional Immobilization of Heparin onto the Nonporous Surface of Polystyrene Microplates
Nadkarni, V.D.; Linhardt, Robert J. (Future Science, 1997)Heparin, a sulfated polysaccharide,exhibits anticoagulant activity mediat-ed through the binding of a specificpentasaccharide sequence in its struc-ture to the protein antithrombin III, acoagulation protease ... -
Directional Immobilization of Heparin to Beaded Supports
Nadkarni, V.D.; Pervin , A.; Linhardt, Robert J. (1994-01-01)Heparin was immobilized in a defined orientation on Sepharose, agarose, and polyacrylamide supports by coupling through its reducing end. This is expected to mimic the attachment of heparin to the protein core in the ... -
Importance of Basic Amino Acids in Heparin and Heparan Sulfate Protein Binding Sites
Caldwell, E.E.O.; Nadkarni, V.D.; Fromm, J.R.; Linhardt, Robert J.; Weiler, J.M. (1996)Heparin and heparan sulfate bind a variety of proteins and peptides to regulate many biological activities. Past studies have examined a limited number of established heparin binding sites and have focused on basic amino ... -
Modified Assay for the Measurement of Biotin in the Presence of Heparin
Nadkarni, V.D.; Linhardt, Robert J. (1995-01-01)Tween 20 is frequently used in blotting analysis of proteins and nucleic acids to decrease background staining due to nonspecific binding of antibodies to various membranes. Inclusion of 0.1% (w/v) Tween 20 in the washing ... -
Preparation and Biological Activity of N-Sulfated Chondroitin and Dermatan Sulfate Derivatives
Nadkarni, V.D.; Toida, T.; VanGorp, C.L.; Schubert, R.L.; Weiler, J.M.; Hansen, K.P.; Caldwell, E.E.O.; Linhardt, Robert J. (1996)Chondroitin and dermatan sulfates are families of structurally complex, sulfated, linear polysaccharides called glycosaminoglycans (GAGs) with alternating uronic acid and N-acetylated D-galactosamine residues [ 1] (Scheme ...