Browsing Linhardt Research Labs Papers by Author "Onishi, Akihiro"

Now showing items 1-8 of 8

    • Addressing endotoxin issues in bioengineered heparin 

      Suwan, Jiraporn; Torelli, Amanda; Onishi, Akihiro; Dordick, Jonathan S.; Linhardt, Robert J. (2012-11-01)
      Heparin is a widely used clinical anticoagulant that is prepared from pig intestine. A contamination of heparin in 2008 has led to a reexamination of animal-derived pharmaceuticals. A bioengineered heparin prepared by ...

    • Analysis of heparins derived from bovine tissues and comparison to porcine intestinal heparins 

      St Ange, Kalib; Onishi, Akihiro; Fu, Li; Sun, Xiaojun; Lin, Lei; Mori, Daisuke; Zhang, Fuming; Dordick, Jonathan S.; Fareed, Jawed; Hoppensteadt, Debra; Jeske, Walter; Linhardt, Robert J. (2016-09-01)
      Heparin is a widely used clinical anticoagulant. It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid ...

    • Combinatorial one-pot chemoenzymatic synthesis of heparin 

      Bhaskar, Ujjwal; Li, Guoyun; Fu, Li; Onishi, Akihiro; Suflita, Mathew; Dordick, Jonathan S.; Linhardt, Robert J. (Elsevier, 2015-05-20)
      Contamination in heparin batches during early 2008 has resulted in a significant effort to develop a safer bioengineered heparin using bacterial capsular polysaccharide heparosan and recombinant enzymes derived from the ...

    • Engineering of routes to heparin and related polysaccharides 

      Bhaskar, Ujjwal; Sterner, Eric; Hickey, Anne Marie; Onishi, Akihiro; Zhang, Fuming; Dordick, Jonathan S.; Linhardt, Robert J. (Springer, 2012-01-01)
      Anticoagulant heparin has been shown to possess important biological functions that vary according to its fine structure. Variability within heparin's structure occurs owing to its biosynthesis and animal tissue-based ...

    • Keratan sulfate glycosaminoglycan from chicken egg white 

      Fu, Li; Sun, Xiaojun; He, Wenqin; Cai, Chao; Onishi, Akihiro; Zhang, Fuming; Linhardt, Robert J.; Liu, Zhangguo (2016-07-01)
      Keratan sulfate (KS) was isolated from chicken egg white in amounts corresponding to ∼0.06 wt% (dry weight). This KS had a weight-average molecular weight of ∼36–41 kDa with a polydispersity of ∼1.3. The primary repeating ...

    • Microscale separation of heparosan, heparan sulfate and heparin 

      Zhao, Xue; Yang, Bo; Linkens, Kathryn; Datta, Payel; Onishi, Akihiro; Zhang, Fuming; Linhardt, Robert J. (Elsevier, 2013-03-15)
      The separation and quantification of glycosaminoglycan (GAG) chains, with different levels of sulfation, from cells, media and prepared through chemoenzymatic synthesis or metabolic engineering, poses a major challenge in ...

    • Structural characterization of pharmaceutical heparins prepared from different animal tissues 

      Fu, Li; Li, Guoyun; Yang, Bo; Onishi, Akihiro; Li, Lingyun; Sun, Peilong; Zhang, Fuming; Linhardt, Robert J. (Elsevier, 2013-01-01)
      Although most pharmaceutical heparin used today is obtained from porcine intestine, heparin has historically been prepared from bovine lung and ovine intestine. There is some regulatory concern about establishing the species ...

    • Structure and activity of a new low molecular weight heparin produced by enzymatic ultrafiltration 

      Fu, Li; Zhang, Fuming; Li, Guoyun; Onishi, Akihiro; Bhaskar, Ujjwal; Sun, Peilong; Linhardt, Robert J. (Elsevier, 2014-01-01)
      The standard process for preparing the low-molecular-weight heparin (LMWH) tinzaparin, through the partial enzymatic depolymerization of heparin, results in a reduced yield because of the formation of a high content of ...