Browsing Linhardt Research Labs Papers by Author "Vaidyanathan, Deepika"

Now showing items 1-4 of 4

    • Elucidating the Unusual Reaction Kinetics of D-Glucuronyl C5-Epimerase 

      Vaidyanathan, Deepika; Paskaleva, Elena; Vargason, Troy; Ke, Xia; Mccallum, Scott A.; Linhardt, Robert J.; Dordick, Jonathan S. (2020-11-01)
      The chemoenzymatic synthesis of heparin, through a multienzyme process, represents a critical challenge in providing a safe and effective substitute for this animal-sourced anticoagulant drug. D-glucuronyl C5-epimerase ...

    • Heavy heparin: A stable isotope-enriched, chemoenzymatically-synthesized, poly-component drug 

      Cress, Brady F.; Bhaskar, Ujjwal; Vaidyanathan, Deepika; Williams, Asher; Cai, Chao; Liu, Xinyue; Fu, Li; M-Chari, Vandhana; Zhang, Fuming; Mousa, Shaker A.; Dordick, Jonathan S.; Koffas, Mattheos A.G.; Linhardt, Robert J. (Wiley, 2019-04-23)
      Heparin is a highly sulfated, complex polysaccharide and widely used anticoagulant pharmaceutical. In this work, we chemoenzymatically synthesized perdeuteroheparin from biosynthetically enriched heparosan precursor obtained ...

    • Metabolic Engineering of Bacillus megaterium for Heparosan Biosynthesis using Pasteurella multocida Heparosan Synthase, PmHS2 

      Williams, Asher; Gedeon, Kamil S.; Vaidyanathan, Deepika; Yu, Yanlei; Collins, Cynthia H.; Dordick, Jonathan S.; Linhardt, Robert J.; Koffas, Mattheos A.G. (Springer Nature, 2019-08-12)
      Background: Heparosan is the unsulfated precursor of heparin and heparan sulfate and its synthesis is typically the first step in the production of bioengineered heparin. In addition to its utility as the starting material ...

    • Polysaccharide Sequence Influences the Specificity and Catalytic Activity of Glucuronyl C5-Epimerase 

      Vaidyanathan, Deepika; Ke, Xia; Yu, Yanlei; Linhardt, Robert J.; Dordick, Jonathan S. (2020-07-14)
      Heparin is a widely used biotherapeutic produced from animal tissues. However, it might be possible to produce a bioengineered version using a multienzyme process, relying on the isolation of the E. coli K5 capsule heparosan ...