Browsing Linhardt Research Labs Papers by Author "Wang, H.M."
Now showing items 1-10 of 10
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Determination of the pKa of Glucuronic Acid and Heparin's Carboxyl Groups by 13C NMR
Wang, H.M.; Loganathan, D.; Linhardt, Robert J. (1991)As part of our continuing studies on heparin, the present paper uses 13C-n.m.r. spectroscopy to examine the acidity of heparin's uronic acid carboxylate groups. Heparin contains three different uronic acids. In porcine ... -
Disaccharide Compositional Analysis of Heparin and Heparan Sulfate Using Capillary Zone Electrophoresis
Ampofo, S.A.; Wang, H.M.; Linhardt, Robert J. (1991-01-01)Capillary zone electrophoresis (CZE) was used to separate eight commercial disaccharide standards of the structure delta UA2X(1----4)-D-GlcNY6X (where delta UA is 4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid, GlcN ... -
Examination of the Substrate Specificity of Heparin and Heparan Sulfate Lyases
Linhardt, Robert J.; Turnbull, J.E.; Wang, H.M.; Loganathan, D.; Gallagher, J.T. (1990-03-01)We have examined the activities of different preparations of heparin and heparan sulfate lyases from Flavobacterium heparinum. The enzymes were incubated with oligosaccharides of known size and sequence and with complex ... -
Glycosaminoglycans Can Influence Fibroblast Growth Factor-2 Mitogenicity Without Significant Growth Factor Binding
Wang, H.M.; Toida, T.; Kim, Y.S.; Capila, I.; Hileman, R.E.; Bernfield, M.; Linhardt, Robert J. (1998-01-06)Fibroblast growth factors are important heparin binding, mitogenic proteins. The binding site in heparin and heparan sulfate for fibroblast growth factor-2 (basic fibroblast growth factor) has been described as rich in ... -
Mapping and Quantification of the Major Oligosaccharide Components of Heparin
Linhardt, Robert J.; Rice, K.G.; Kim, Y.S.; Lohse, D.L.; Wang, H.M.; Loganathan, D. (1988-01-01)A new method of determining the oligosaccharide composition of commercial glycosaminoglycan heparin is described in which heparin was first depolymerized using heparin lyase (EC 4.2.2.7), and then analysed by a single ... -
New Methodologies in Heparin Structure Analysis and the Generation of Low Molecular Weight Heparins
Linhardt, Robert J.; Wang, H.M.; Ampofo, S.A. (1992)This chapter examines the methods used to prepare heparin, low molecular weight (LMW) heparins, and immobilized heparins. These heparins and heparin derivatives arc polydispcrse heterogeneous mixtures that require structural ... -
Oligosaccharide Compositional Analysis of Heparin and Low Molecular Weight Heparins by Capillary Electrophoresis
Desai, U.R.; Wang, H.M.; Ampofo, S.A.; Linhardt, Robert J. (1993)The application of capillary electrophoresis to total compositional analysis of heparin and low-molecular-weight heparin samples has been studied. Optimum resolution of 17 defined oligosaccharides was obtained with the ... -
Oligosaccharide Mapping of Low Molecular Weight Heparins: Structural Differences and their Relationship to Activity
Linhardt, Robert J.; Loganathan, D.; Al-Hakim, A.; Wang, H.M.; Walenga, J.M.; Hoppensteadt, D.; Fareed, J. (1990)Low molecular weight heparins from a variety of commercial sources were examined. These had been prepared by several methods including peroxidative cleavage, nitrous acid cleavage, chemical beta-elimination, enzymatic ... -
Search for the Heparin Antithrombin III Binding Site Precursor
Linhardt, Robert J.; Wang, H.M.; Loganathan, D.; Bae, J.H. (Elsevier, 1992)The last step of heparin biosynthesis is thought to involve the action of 3-O-sulfotransferase resulting in the formation of an antithrombin III (ATIII) binding site required for heparin's anticoagulant activity. The ... -
Search for the Heparin Antithrombin III Binding Site Precursor
Linhardt, Robert J.; Wang, H.M.; Loganathan, D.; Bae, J.H. (Elsevier, 1992)The last step of heparin biosynthesis is thought to involve the action of 3-O-sulfotransferase resulting in the formation of an antithrombin III (ATIII) binding site required for heparin's anticoagulant activity. The ...