Browsing Linhardt Research Labs Papers by Author "Xia, K."
Now showing items 1-10 of 10
-
Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices
Silva, J.C.; Carvalho, M.S.; Han, X.; Xia, K.; Mikael, P.E.; Cabral, J.M.S.; Castelo Ferreira, F.; Linhardt, Robert J. (Springer, 2019-04-15)The extracellular matrix (ECM) is a highly dynamic and complex meshwork of proteins and glycosaminoglycans (GAGs) with a crucial role in tissue homeostasis and organization not only by defining tissue architecture and ... -
Effect of high glucose on glycosaminoglycans in cultured retinal endothelial cells and rat retina
Kaur, G.; Song, Y.; Xia, K.; McCarthy, K.; Zhang, F.; Linhardt, Robert J.; Harris, N.R. (2022)Introduction The endothelial glycocalyx regulates vascular permeability, inflammation, and coagulation, and acts as a mechanosensor. The loss of glycocalyx can cause endothelial injury and contribute to several microvascular ... -
Enzymatic synthesis of chondroitin sulfate E to attenuate bacterial lipopolysaccharide-induced organ damage
Li, J.; Sparkenbaugh, E.; Su, G.; Zhang, F.; Xu, Y.; Xia, K.; He, P.; Baytas, S.; Pechauer, S.; Padmanabhan, A.; Linhardt, Robert J.; Pawlinski, R.; Liu, J. (American Chemical Society (ACS), 2020)Chondroitin sulfate E (CS-E) is a sulfated polysaccharide that contains repeating disaccharides of 4,6-disulfated N-acetylgalactosamine and glucuronic acid residues. Here, we report the enzymatic synthesis of three homogeneous ... -
Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry
Silva, J.C.; Carvalho, M.S.; Xia, K.; Cabral, J.M.S.; da Silva, C.L.; Ferreira, F.C.; Vashishth, D.; Linhardt, Robert J. (2020-01-01)Cell-derived extracellular matrices have emerged as promising scaffolds for tissue engineering (TE) strategies due to their ability to create a biomimetic microenvironment providing biochemical and physical cues to cells, ... -
Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow−/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia
Silva, J.C.; Han, X.; Silva, T.P.; Xia, K.; Mikael, P.E.; Cabral, J.M.S.; Ferreira, F.C.; Linhardt, Robert J. (2020-06-01)Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators ... -
Increased 3’-phosphoadenosine-5’-phosphosulfate levels in engineered Escherichia coli cell lysate facilitates in vitro synthesis of chondroitin sulfate A
Badri, A.; Williams, A.; Xia, K.; Linhardt, Robert J.; Koffas, M.A.G. (Wiley, 2019)Chondroitin sulfates (CSs) are linear glycosaminoglycans that have important applications in the medical and food industries. Engineering bacteria for the microbial production of CS will facilitate a one-step, scalable ... -
Increased CHST15 Expression follows Decline in Arylsulfatase B (ARSB) and Disinhibition of Non-Canonical WNT Signaling: Potential for Epithelial-Mesenchymal Transition
Bhattacharyya, S.; Feferman, L.; Han, X.; Xia, K.; Zhang, F.; Linhardt, Robert J.; Tobacman, J.K. (Impact Journals, 2020)Expression of CHST15 (carbohydrate sulfotransferase 15; chondroitin 4-sulfate-6-sulfotransferase; BRAG), the sulfotransferase enzyme that adds 6-sulfate to chondroitin 4-sulfate (C4S) to make chondroitin 4,6-disulfate ... -
Lipase Catalyzed Synthesis and Characterization of Poly (Glycerol-Sebacate)
Ning, Z.; Lang, K.; Xia, K.; Linhardt, Robert J.; Gross, R. (2022)This study demonstrated that immobilized Candida antarctica lipase B (N435) catalysis in bulk leads to higher molecular weight poly(glycerol sebacate), PGS, than self-catalyzed condensation polymerization. Since the ... -
A revised structure for the glycolipid terminus of E. coli K5 heparosan capsular polysaccharide
Yan, L.; Fu, L.; Xia, K.; Chen, S.; Zhang, F.; Dordick, J.S.; Linhardt, Robert J. (Multidisciplinary Digital Publishing Institute (MDPI), 2020)The structure of heparosan capsular polysaccharide (CPS) has been determined using enzymatic digestion with nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Previous errors in the assignment of the ... -
The effect of electrospun scaffolds on the glycosaminoglycan profile of differentiating neural stem cells
Garrudo, F.F.F.; Mikael, P.E.; Xia, K.; Silva, J.C.; Ouyang, Y.; Chapman, C.A.; Hoffman, P.R.; Yu, Y.; Han, X.; Rodrigues, C.A.V.; Cabral, J.M.S.; Morgado, J.; Ferreir F.C.; Linhardt, Robert J. (Elsevier, 2021-03-01)The use of electrospun scaffolds for neural tissue engineering applications allows a closer mimicry of the native tissue extracellular matrix (ECM), important for the transplantation of cells in vivo. Moreover, the role ...