Browsing Linhardt Research Labs Papers by Author "Xia, Ke"

Now showing items 1-20 of 26

    • Bottom-up and top-down profiling of pentosan polysulfate 

      Lin, Lei; Yu, Yanlei; Zhang, Fuming; Xia, Ke; Zhang, Xing; Linhardt, Robert J. (Royal Society of Chemistry, 2019-08-21)
      Pentosan polysulfate (PPS) is a semi-synthetic glycosaminoglycan (GAG) mimetic. PPS, synthesized through the chemical sulfonation of a plant-derived β-(1 → 4)-xylan, is the active pharmaceutical ingredient of the drug ...

    • Chemobiocatalytic Synthesis of a Low Molecular Weight Heparin 

      Yu, Yanlei; Fu, Li; He, Peng; Xia, Ke; Varghese, Sony; Wang, Hong; Zhang, Fuming; Dordick, Jonathan; Linhardt, Robert J. (2022-03-18)
      Heparin products are widely used clinical anticoagulants essential in the practice of modern medicine. Low-molecular-weight heparins (LMWHs) are currently prepared by the controlled chemical or enzymatic depolymerization ...

    • Chemometric analysis of porcine, bovine and ovine heparins 

      Ouyang, Yilan; Han, Xiaorui; Yu, Yanlei; Chen, Jianle; Fu, Li; Zhang, Fuming; Linhardt, Robert J.; Fareed, Jawed; Hoppensteadt, Debra; Jeske, Walter; Kouta, Ahmed; Zhang, Zhenqing; Xia, Ke (2019-02-05)
      Heparin is a polysaccharide anticoagulant drug isolated from animal tissues. There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption. ...

    • Circulating heparin oligosaccharides rapidly target the hippocampus in sepsis potentially impacting cognitive functions 

      Zhang, Xing; Han, Xiaorui; Xia, Ke; Xu, Yongmei; Yang, Yimu; Oshima, Kaori; Haeger, Sarah M.; Perez, Mario J.; McMurtry, Sarah A.; Hippensteel, Joseph A.; Ford, Joshay A.; Herson, Paco S.; Liu, Jian; Schmidt, Eric P.; Linhardt, Robert J. (2019-05-07)
      Sepsis induces heparanase-mediated degradation of the endothelial glycocalyx, a heparan sulfate-enriched endovascular layer critical to vascular homeostasis, releasing highly sulfated domains of heparan sulfate into the ...

    • Comparison of low-molecular-weight heparins prepared from ovine heparins with enoxaparin 

      Chen, Jianle; Yu, Yanlei; Fareed, Jawed; Hoppensteadt, Debra; Jeske, Walter; Kouta, Ahmed; Jin, Caijuan; Jin, Yongsheng; Yao, Yiming; Xia, Ke; Zhang, Fuming; Chen, Shiguo; Ye, Xingqian; Linhardt, Robert J. (Sage, 2019-04-12)
      Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants. These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, ...

    • Complete biosynthesis of a sulfated chondroitin in Escherichia coli 

      Badri, Abinaya; Williams, Asher; Awofiranye, Adeola; Datta, Payel; Xia, Ke; He, Wenqin; Fraser, Keith; Dordick, Jonathan S.; Linhardt, Robert J.; Koffas, Mattheos A.G. (Nature, 2021-12-01)
      Sulfated glycosaminoglycans (GAGs) are a class of important biologics that are currently manufactured by extraction from animal tissues. Although such methods are unsustainable and prone to contamination, animal-free ...

    • Design of anti-inflammatory heparan sulfate to protect against acetaminophen-induced acute liver failure 

      Arnold, Katelyn; Xu, Yongmei; Sparkenbaugh, Erica M.; Li, Miaomiao; Han, Xiaorui; Zhang, Xing; Xia, Ke; Piegore, Mark; Zhang, Fuming; Zhang, Xiaoxiao; Henderson, Mike; Pagadala, Vijayakanth; Su, Guowei; Tan, Lisi; Park, Pyong Woo; Stravitz, Richard T.; Key, Nigel S.; Linhardt, Robert J.; Pawlinski, Rafal; Xu, Ding; Liu, Jian (American Association for the Advancement of Science (AAAS), 2020-03-18)
      Acetaminophen/paracetamol (APAP) overdose is the leading cause of drug-induced acute liver failure (ALF) in the United States and Europe. The progression of the disease is attributed to sterile inflammation induced by the ...

    • Fractionation of sulfated galactan from the red alga Botryocladia occidentalis separates its anticoagulant and anti-SARS-CoV-2 properties 

      Kim, Seon Beom; Zoepfl, Mary; Samanta, Priyanka; Zhang, Fuming; Xia, Ke; Thara, Reena; Linhardt, Robert J.; Doerksen, Robert J.; McVoy, Michael A.; Pomin, Vitor H. (Elsevier, 2022-05-01)
      Sulfation pattern and molecular weight (MW) play a key role in the biological actions of sulfated glycans. Besides anticoagulant effects, certain sulfated glycans can also exhibit anti-SARS-CoV-2 properties. To develop a ...

    • Functional role of glycosaminoglycans in decellularized lung extracellular matrix 

      Uhl, Franziska E.; Zhang, Fuming; Pouliot, Robert A.; Uriarte, Juan J.; Rolandsson Enes, Sara; Han, Xiaorui; Ouyang, Yilan; Xia, Ke; Westergren-Thorsson, Gunilla; Malmström, Anders; Hallgren, Oskar; Linhardt, Robert J.; Weiss, Daniel J. (Elsevier, 2020-01-15)
      Despite progress in use of decellularized lung scaffolds in ex vivo lung bioengineering schemes, including use of gels and other materials derived from the scaffolds, the detailed composition and functional role of ...

    • Glycosaminoglycans in human cerebrospinal fluid determined by LC-MS/MS MRM 

      Yu, Yanlei; Zhang, Fuming; Colón, Wilfredo; Linhardt, Robert J.; Xia, Ke (Elsevier, 2019-02-15)
      Glycosaminoglycans (GAGs) were recovered from human cerebral spinal fluid (CSF) and after their conversion to disaccharides using polysaccharide lyases were analyzed by liquid chromatography tandem mass spectrometry using ...

    • GRASP depletion-mediated Golgi fragmentation impairs glycosaminoglycan synthesis, sulfation, and secretion 

      Ahat, Erpan; Song, Yuefan; Xia, Ke; Reid, Whitney; Li, Jie; Bui, Sarah; Zhang, Fuming; Linhardt, Robert J.; Wang, Yanzhuang (Springer, 2022-04-01)
      Synthesis of glycosaminoglycans, such as heparan sulfate (HS) and chondroitin sulfate (CS), occurs in the lumen of the Golgi, but the relationship between Golgi structural integrity and glycosaminoglycan synthesis is not ...

    • Heparan sulfates from bat and human lung and their binding to the spike protein of SARS-CoV-2 virus 

      Yan, Lufeng; Song, Yuefan; Xia, Ke; He, Peng; Zhang, Fuming; Chen, Shiguo; Pouliot, Robert; Weiss, Daniel J.; Tandon, Ritesh; Bates, John T.; Ederer, Dallas R.; Mitra, Dipanwita; Sharma, Poonam; Davis, April; Linhardt, Robert J. (2021-05-15)
      Severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has resulted in a pandemic and continues to spread at an unprecedented rate around the world. Although a vaccine has recently been approved, there are ...

    • Interactions of Fibroblast Growth Factors with Sulfated Galactofucan from Saccharina japonica 

      Jin, Weihua; Jiang, Di; Zhang, Wenjing; Wang, Chunyu; Xia, Ke; Zhang, Fuming; Linhardt, Robert J. (2020-10-01)
      A total 68 types of marine algae oligosaccharides and polysaccharides were prepared and used to study the structure-activity relationship of oligosaccharides and polysaccharides in their interactions with fibroblast growth ...

    • Metabolic engineering of cyanobacteria for photoautotrophic production of heparosan, a pharmaceutical precursor of heparin 

      Sarnaik, Aditya; Abernathy, Mary H.; Han, Xiaorui; Ouyang, Yilan; Xia, Ke; Chen, Yin; Cress, Brady; Zhang, Fuming; Lali, Arvind; Pandit, Reena; Linhardt, Robert J.; Tang, Yinjie J.; Koffas, Mattheos A.G. (2019-01-01)
      Heparosan is an unsulfated polysaccharide potentially important for its wide range of cosmetic and pharmaceutical applications, particularly as the precursor for the extensively used anticoagulant, heparin. Generally sourced ...

    • N-glycolyl chondroitin synthesis using metabolically engineered E. coli 

      Awofiranye, Adeola E.; Baytas, Sultan N.; Xia, Ke; Badri, Abinaya; He, Wenqin; Varki, Ajit; Koffas, Mattheos; Linhardt, Robert J. (Springer, 2020)
      N-glycolyl chondroitin (Gc-CN) is a metabolite of N-glycolylneuraminic acid (Neu5Gc), a sialic acid that is commonly found in mammals, but not humans. Humans can incorporate exogenous Neu5Gc into their tissues from eating ...

    • Negative-ion mode capillary isoelectric focusing-mass spectrometry for charge-based separation of acidic oligosaccharides 

      Ouyang, Yilan; Han, Xiaorui; Xia, Qiangwei; Chen, Jianle; Velagapudi, Sheila; Xia, Ke; Zhang, Zhenqing; Linhardt, Robert J. (2019-01-02)
      Glycosaminoglycans (GAGs) are biologically and pharmacologically important linear, anionic polysaccharides containing various repeating disaccharides sequences. The analysis of these polysaccharides generally relies on ...

    • Non-anticoagulant low molecular weight heparins for pharmaceutical applications 

      Ouyang, Yilan; Yu, Yanlei; Zhang, Fuming; Chen, Jianle; Han, Xiaorui; Xia, Ke; Yao, Yiming; Zhang, Zhenqing; Linhardt, Robert J. (2019-01-24)
      Heparin is a polypharmacological agent with anticoagulant activity. Periodate oxidation of the nonsulfated glucuronic acid residue results in non-anticoagulant heparin derivative (NACH) of reduced molecular weight. Similar ...

    • Preparation of low molecular weight heparin from a remodeled bovine intestinal heparin 

      Baytas, Sultan N.; Varghese, Sony S.; Jin, Weihua; Yu, Yanlei; He, Peng; Douaisi, Marc; Zhang, Fuming; Brodfuehrer, Paul; Xia, Ke; Dordick, Jonathan S.; Linhardt, Robert J. (2021-02-25)
      Bovine intestinal heparins are structurally distinct from porcine intestinal heparins and exhibit lower specific anticoagulant activity (units/mg). The reduced content of N-sulfo, 3-O-sulfo glucosamine, the central and ...

    • Recent advances in biotechnology for heparin and heparan sulfate analysis 

      Qiao, Meng; Lin, Lei; Xia, Ke; Lid, Jun; Zhang, Xing; Linhardt, Robert J. (Elsevier, 2020-11-01)
      Heparan sulfate (HS) is a class of linear, sulfated, anionic polysaccharides, called glycosaminoglycans (GAGs), which present on the mammalian cell surfaces and extracellular matrix. HS GAGs display a wide range of critical ...

    • Remodeling of Glycosaminoglycans During Differentiation of Adult Human Bone Mesenchymal Stromal Cells toward Hepatocytes 

      Mikael, Paiyz E.; Willard, Charles; Koyee, Aurvan; Barlao, Charmaine Grace; Liu, Xinyue; Han, Xiaorui; Ouyang, Yilan; Xia, Ke; Linhardt, Robert J.; Dordick, Jonathan S. (2019-02-15)
      There is a critical need to generate functional hepatocytes to aid in liver repair and regeneration upon availability of a renewable, and potentially personalized, source of human hepatocytes (hHEP). Currently, the vast ...