Browsing Linhardt Research Labs Papers by Author "Xie, Jin"

Now showing items 1-11 of 11

    • Changes in cultured endothelial cell glycosaminoglycans under hyperglycemic conditions and the effect of insulin and heparin 

      Han, Juying; Zhang, Fuming; Xie, Jin; Linhardt, Robert J.; Hiebert, Linda M. (Springer Nature, 2009-08-20)
      Background: Heparan sulfate proteoglycans (HSPGs) contain glycosaminoglycan (GAG) chains made primarily of heparan sulfate (HS). Hyperglycemia in diabetes leads to endothelial injury and nephropathy, retinopathy and ...

    • Glycomics of Proteoglycan Biosynthesis in Murine Embryonic Stem Cell Differentiation 

      Nairn, Alison V.; Kinoshita-Toyoda, Akiko; Toyoda, Hidenao; Xie, Jin; Harris, Kyle; Dalton, Stephen; Kulik, Michael; Pierce, J. Michael; Toida, Toshihiko; Moremen, Kelley W.; Linhardt, Robert J. (American Chemical Society (ACS), 2007-11-01)
      Glycosaminoglycans (GAGs) play a critical role in binding and activation of growth factors involved in cell signaling critical for developmental biology. The biosynthetic pathways for GAGs have been elucidated over the ...

    • Immobilization of Heparin-Approaches and Applications 

      Murugesan, Saravanababu; Xie, Jin; Linhardt, Robert J. (Bentham Science, 2008-01-01)
      Heparin, an anticoagulant, has been used in many forms to treat various diseases. These forms include soluble heparin and heparin immobilized to supporting matrices by physical adsorption, by covalent chemical methods and ...

    • Increase in the Growth Inhibition of Bovine Pulmonary Artery Smooth Muscle Cells by an O-Hexanoyl Low Molecular Weight Heparin Derivative 

      Garg, Hari G.; Hales, Charles A.; Yu, Lunyin; Butler, Melissa; Islam, Tasneem; Xie, Jin; Linhardt, Robert J. (Elsevier, 2006-11-06)
      Proliferation of pulmonary artery smooth muscle cells (PASMCs) appears to play a significant role in chronic pulmonary hypertension. The proliferation of PASMCs is strongly inhibited by some commercial heparin preparations. ...

    • Isolation and Characterization of Heparan Sulfate from Various Murine Tissues 

      Warda, Mohamad; Toida, Toshihiko; Zhang, Fuming; Sun, Peilong; Munoz, Eva; Xie, Jin; Linhardt, Robert J. (Springer, 2006-11-01)
      Heparan sulfate (HS), is a proteoglycan (PG) found both in the extracellular matrix and on cell surface. It may represent one of the most biologically important glycoconjugates, playing an essential role in a variety of ...

    • Mosquito Heparan Sulfate and its Potential Role in Malaria Infection and Transmission 

      Sinnis, Photini; Coppi, Alida; Toida, Toshihiko; Toyoda, Hidenao; Kinoshita-Toyoda, Akiko; Xie, Jin; Kemp, Melissa M.; Linhardt, Robert J. (Elsevier, 2007-08-31)
      Heparan sulfate has been isolated for the first time from the mosquito Anopheles stephensi, a known vector for Plasmodium parasites, the causative agents of malaria. Chondroitin sulfate, but not dermatan sulfate or hyaluronan, ...

    • Physiological, Pathophysiological and Therapeutic Roles of Heparin/Heparan Sulfate 

      Xie, Jin; Murugesan, Saravanababu; Linhardt, Robert J. (2008-12-01)
      Heparin and heparan sulfate belong to the glycosaminoglycan (GAG) family of carbohydrates. They are linear acidic complex polysaccharides found on the cell surface and in the extracellular matrix. Heparin and heparan sulfate ...

    • Solution Structures of Chemoenzymatically Synthesized Heparin and its Precursors 

      Zhang, Zhenqing; McCallum, Scott A.; Xie, Jin; Nieto, Lidia; Corzana, Francisco; Jiménez-Barbero, Jesús; Chen, Miao; Liu, Jian; Linhardt, Robert J. (American Chemical Society (ACS), 2008-10-01)
      We report the first chemoenzymatic synthesis of the stable isotope-enriched heparin from a uniformly labeled [(13)C,(15)N]N-acetylheparosan (-GlcA(1,4)GlcNAc-) prepared from E. coli K5. Glycosaminoglycan (GAG) precursors ...

    • Structural Analysis of Bikunin Glycosaminoglycan 

      Chi, Lianli; Wolff, Jeremy J.; Laremore, Tatiana N.; Restaino, Odile Francesca; Xie, Jin; Schiraldi, Chiara; Toida, Toshihiko; Amster, I. Jonathan; Linhardt, Robert J. (American Chemical Society (ACS), 2008-02-27)
      The structure of an intact glycosaminoglycan (GAG) chain of the bikunin proteoglycan (PG) was analyzed using a combined top-down and bottom-up sequencing strategy. PGs are proteins with one or more linear, high-molecular ...

    • Tandem MS Can Distinguish Hyaluronic Acid from N-Acetylheparosan 

      Zhang, Zhenqing; Xie, Jin; Liu, Jian; Linhardt, Robert J. (2008-01-01)
      Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was ...

    • Thin Layer Chromatography for the Analysis of Glycosaminoglycan Oligosaccharides 

      Zhang, Zhenqing; Xie, Jin; Zhang, Fuming; Linhardt, Robert J. (Elsevier, 2007-12-01)
      Thin layer chromatography was used to analyze glycosaminoglycan oligosaccharides obtained through the use of polysaccharide lyases. This method allows for the rapid, semi-quantitative analysis of a wide variety of ...