Browsing Linhardt Research Labs Papers by Author "Yang, Bo"
Now showing items 1-20 of 25
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Analysis of 3-O-sulfo group containing heparin tetrasaccharides in heparin by liquid chromatography-mass spectrometry
Li, Guoyun; Yang, Bo; Li, Lingyun; Zhang, Fuming; Xue, Changhu; Linhardt, Robert J. (Elsevier, 2014-06-15)Complete heparin digestion with heparin lyase 2 affords a mixture of disaccharides and resistant tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends. Quantitative online liquid ... -
Analysis of glycosaminoglycan-derived disaccharides by capillary electrophoresis using laser-induced fluorescence detection
Chang, Yuqing; Yang, Bo; Zhao, Xue; Linhardt, Robert J. (Elsevier, 2012-08-01)A quantitative and highly sensitive method for the analysis of glycosaminoglycan (GAG)-derived disaccharides is presented that relies on capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection. This ... -
Bioengineered Chinese hamster ovary cells with Golgi-targeted 3-O-sulfotransferase-1 biosynthesize heparan sulfate with an antithrombin binding site
Datta, Payel; Li, Guoyun; Yang, Bo; Zhao, Xue; Baik, Jong Youn; Gemmill, Trent R.; Sharfstein, Susan T.; Linhardt, Robert J. (The American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier, 2013-12-27)HS3st1 (heparan sulfate 3-O-sulfotransferase isoform-1) is a critical enzyme involved in the biosynthesis of the antithrombin III (AT)-binding site in the biopharmaceutical drug heparin. Heparin is a highly sulfated ... -
Bioengineering murine mastocytoma cells to produce anticoagulant heparin
Gasimli, Leyla; Glass, Charles A.; Datta, Payel; Yang, Bo; Li, Guoyun; Gemmill, Trent R.; Baik, Jong Youn; Sharfstein, Susan T.; Esko, Jeffrey D.; Linhardt, Robert J. (2014-03-01)Heparin (HP), an important anticoagulant polysaccharide, is produced in a complex biosynthetic pathway in connective tissue-type mast cells. Both the structure and size of HP are critical factors determining the anticoagulation ... -
Capillary electrophoresis for the analysis of glycosaminoglycan-derived disaccharides
Chang, Yuqing; Yang, Bo; Weyers, Amanda; Linhardt, Robert J. (2013-01-01)A quantitative and highly sensitive method for the analysis of glycosaminoglycan (GAG)-derived disaccharides is presented that relies on capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection. This ... -
Cell-based microscale isolation of glycoaminoglycans for glycomics study
Zhao, Xue; Yang, Bo; Datta, Payel; Gasmili, Leyla; Zhang, Fuming; Linhardt, Robert J. (Taylor and Francis, 2012-07-09)Glycomics research requires the isolation of glycans from cells for structural characterization and functional studies of the glycans. A method for cell-based microscale isolation and quantification of highly sulfated, ... -
Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages
Gasimli, Leyla; Hickey, Anne Marie; Yang, Bo; Li, Guoyun; Dela Rosa, Mitche; Nairn, Alison V.; Kulik, Michael J.; Dordick, Jonathan S.; Moremen, Kelley W.; Dalton, Stephen; Linhardt, Robert J. (Elsevier, 2014-01-01)Background: Proteoglycans are found on the cell surface and in the extracellular matrix, and serve as prime sites for interaction with signaling molecules. Proteoglycans help regulate pathways that control stem cell fate, ... -
Characterization of human placental glycosaminoglycans and regional binding to VAR2CSA in malaria infected erythrocytes
Beaudet, Julie M.; Mansur, Leandra; Joo, Eun Ji; Kamhi, Eyal; Yang, Bo; Clausen, Thomas M.; Salanti, Ali; Zhang, Fuming; Linhardt, Robert J. (Springer, 2014-02-01)Placental malaria is a serious problem in sub-Saharan Africa. Young women are particular susceptible to contracting this form of malaria during their first or second pregnancy despite previously acquired immunity from past ... -
Control of the heparosan N-deacetylation leads to an improved bioengineered heparin
Wang, Zhenyu; Yang, Bo; Zhang, Zhenqing; Ly, Mellisa; Takieddin, Majde; Mousa, Shaker; Liu, Jian; Dordick, Jonathan S.; Linhardt, Robert J. (Springer, 2011-07-01)The production of the anticoagulant drug heparin from non-animal sources has a number of advantages over the current commercial production of heparin. These advantages include better source material availability, improved ... -
Exposure to common food additive carrageenan leads to reduced sulfatase activity and increase in sulfated glycosaminoglycans in human epithelial cells
Yang, Bo; Bhattacharyya, Sumit; Linhardt, Robert J.; Tobacman, Joanne (Elsevier, 2012-06-01)The commonly used food additive carrageenan, including lambda (λ), kappa (κ) and iota (ι) forms, is composed of galactose disaccharides linked in alpha-1,3 and beta-1,4 glycosidic bonds with up to three sulfate groups per ... -
Hyphenated techniques for the analysis of heparin and heparan sulfate
Yang, Bo; Solakyildirim, Kemal; Chang, Yuqing; Linhardt, Robert J. (Springer, 2011-01-01)The elucidation of the structure of glycosaminoglycan has proven to be challenging for analytical chemists. Molecules of glycosaminoglycan have a high negative charge and are polydisperse and microheterogeneous, thus ... -
Impact of autoclave sterilization on the activity and structure of formulated heparin
Beaudet, Julie M.; Weyers, Amanda; Solakyildirim, Kemal; Yang, Bo; Takieddin, Majde; Mousa, Shaker; Zhang, Fuming; Linhardt, Robert J. (Elsevier, 2011-01-01)The stability of a formulated heparin was examined during its sterilization by autoclaving. A new method to follow loss in heparin binding to the serine protease inhibitor, antithrombin III, and the serine protease, thrombin, ... -
Isolation of bovine corneal keratan sulfate and its growth factor and morphogen binding
Weyers, Amanda; Yang, Bo; Solakyildirim, Kemal; Yee, Vienna; Li, Lingyun; Zhang, Fuming; Linhardt, Robert J. (FEBS Press, 2013-05-01)Keratan sulfate (KS) is an important glycosaminoglycan that is found in cartilage, reproductive, and neural tissues. Corneal KS glycosaminoglycan is found N-linked to lumican, keratocan, and mimecan proteoglycans and has ... -
Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin
Baik, Jong Youn; Gasimli, Leyla; Yang, Bo; Datta, Payel; Zhang, Fuming; Glass, Charles A.; Esko, Jeffrey D.; Linhardt, Robert J.; Sharfstein, Susan T. (Elsevier, 2012-03-01)Heparin is the most widely used pharmaceutical to control blood coagulation in modern medicine. A health crisis that took place in 2008 led to a demand for production of heparin from non-animal sources. Chinese hamster ... -
Microanalysis of stomach cancer glycosaminoglycans
Weyers, Amanda; Yang, Bo; Park, Jong Hwan; Kim, Yong Seok; Kim, Sun Moon; Lee, Sang Eok; Zhang, Fuming; Lee, Kyung Bok; Linhardt, Robert J. (Springer, 2013-10-01)Glycosaminoglycans (GAGS) are anionic, linear, polysaccharides involved in cell signaling. The GAG content, composition and structure of human tissue have been suggested to play a role in cancer and might provide useful ... -
Microscale separation of heparosan, heparan sulfate and heparin
Zhao, Xue; Yang, Bo; Linkens, Kathryn; Datta, Payel; Onishi, Akihiro; Zhang, Fuming; Linhardt, Robert J. (Elsevier, 2013-03-15)The separation and quantification of glycosaminoglycan (GAG) chains, with different levels of sulfation, from cells, media and prepared through chemoenzymatic synthesis or metabolic engineering, poses a major challenge in ... -
Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells
Datta, Payel; Yang, Bo; Linhardt, Robert J.; Sharfstein, Susan T. (2015-03-18)Sodium butyrate, a histone deacetylase inhibitor, has been used to improve transgene expression in Chinese hamster ovary (CHO) cells. The current study explores the impact of butyrate treatment on heparan sulfate (HS) ... -
On-line separation and characterization of hyaluronan oligosaccharides derived from radical depolymerization
Zhao, Xue; Yang, Bo; Li, Lingyun; Zhang, Fuming; Linhardt, Robert J. (Elsevier, 2013-01-01)Hydroxyl radicals are widely implicated in the oxidation of carbohydrates in biological and industrial processes and are often responsible for their structural modification resulting in functional damage. In this study, ... -
Optimization of bioprocess conditions improves production of a CHO cell-derived, bioengineered heparin
Baik, Jong Youn; Dahodwala, Hussain; Oduah, Eziafa; Talman, Lee; Gemmill, Trent R.; Gasimli, Leyla; Datta, Payel; Yang, Bo; Li, Guoyun; Zhang, Fuming; Li, Lingyun; Linhardt, Robert J.; Campbell, Andrew M.; Gorfien, Stephen F.; Sharfstein, Susan T. (Wiley, 2015-07-01)Heparin is the most widely used anticoagulant drug in the world today. Heparin is currently produced from animal tissues, primarily porcine intestines. A recent contamination crisis motivated development of a non-animal-derived ... -
Photochemical preparation of a novel low molecular weight heparin
Higashi, Kyohei; Hosoyama, Saori; Ohno, Asami; Masuko, Sayaka; Yang, Bo; Sterner, Eric; Wang, Zhenyu; Linhardt, Robert J.; Toida, Toshihiko (Elsevier, 2012-01-15)Commercial low molecular weight heparins (LMWHs) are prepared by several methods including peroxidative cleavage, nitrous acid cleavage, chemical ß-elimination, and enzymatic β-elimination. The disadvantages of these methods ...