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dc.rights.licenseCC BY — Creative Commons Attribution
dc.contributor.authorLamkin, Elliott
dc.contributor.authorCheng, Georgiana
dc.contributor.authorCalabro, Anthony
dc.contributor.authorHascall, Vincent C.
dc.contributor.authorJoo, Eun Ji
dc.contributor.authorLi, Lingyun
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorLauer, Mark E.
dc.date2015
dc.date.accessioned2022-06-20T16:30:41Z
dc.date.available2022-06-20T16:30:41Z
dc.date.issued2015-02-20
dc.identifier.citationHeavy chain transfer by tumor-necrosis-factor-stimulated-gene-6 to the bikunin proteoglycan, E. Lamkin, A. Calabro, V. C. Hascall, E. J. Joo, L. Li, R. J. Linhardt, M.E. Lauer, Journal of Biological Chemistry, 290, 5156–5166, 2015.
dc.identifier.issn1083351X
dc.identifier.issn219258
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5016
dc.identifier.urihttps://doi.org/10.1074/jbc.M114.636258
dc.descriptionJournal of Biological Chemistry, 290, 5156–5166
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractWe present data that hyaluronan (HA) polysaccharides, about 14-86 monosaccharides in length, are capable of accepting only a single heavy chain (HC) from inter-α-inhibitor via transfer by tumor necrosis factor-stimulated gene 6 (TSG-6) and that this transfer is irreversible. We propose that either the sulfate groups (or the sulfation pattern) at the reducing end of the chondroitin sulfate (CS) chain of bikunin, or the core protein itself, enables the bikunin proteoglycan (PG) to accept more than a single HC and permits TSG-6 to transfer these HCs from its relatively small CS chain to HA. To test these hypotheses, we investigated HC transfer to the intact CS chain of the bikunin PG, and to the free chain of bikunin. We observed that both the free CS chain and the intact bikunin PG were only able to accept a single HC from inter-α-inhibitor via transfer by TSG-6 and that HCs could be swapped from the bikunin PG and its free CS chain to HA. Furthermore, a significant portion of the bikunin PG was unable to accept a single heavy chain. We discuss explanations for these observations, including the intracellular assembly of inter-α-inhibitor. In summary, these data demonstrate that the sulfation of the CS chain of bikunin and/or its core protein promote HC transfer by TSG-6 to its relatively short CS chain, although they are insufficient to enable the CS chain of bikunin to accept more than one HC in the absence of other cofactors.
dc.description.sponsorshipNational Institutes of Health
dc.languageen_US
dc.language.isoENG
dc.publisherThe American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofJournal of Biological Chemistry
dc.relation.urihttps://harc.rpi.edu/
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleHeavy chain transfer by tumor-necrosis-factor-stimulated-gene-6 to the bikunin proteoglycanen_US
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.accessRightsOpen Access
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1074/jbc.M114.636258
dc.rights.holderCC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.; Attribution 3.0 United States
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages5156-5166
rpi.description.volume290


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