Identification of Keratan Sulfate Disaccharide Unit at the C-3 position of Glucuronic Acid of Chondroitin Sulfate from Mactra chinensis
AuthorHigashi, K.; Takeda, Keita; Mukuno, A.; Okamoto, Y.; Masuko, S.; Linhardt, Robert J.; Toida, T.
SubjectBiology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Full CitationIdentification of Keratan Sulfate Disaccharide Unit at the C-3 position of Glucuronic Acid of Chondroitin Sulfate from Mactra chinensis, K. Higashi, K. Takeda, A. Mukuno, Y. Okamoto, S. Masuko, R. J. Linhardt, T. Toida, Biochemical Journal, 473 4145–4158, 2016.
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AbstractGlycosaminoglycans (GAGs), including chondroitin sulfate (CS), dermatan sulfate, heparin, heparan sulfate and keratan sulfate (KS) are linear sulfated repeating disaccharide sequences containing hexosamine and uronic acid [or galactose (Gal) in the case of KS]. Among the GAGs, CS shows structural variations, such as sulfation patterns and fucosylation, which are responsible for their physiological functions through CS interaction with CS-binding proteins. Here, we solved the structure of KS-branched CS-E derived from a clam, Mactra chinensis. KS disaccharide [d-GlcNAc6S-(1→3)-β-d-Gal-(1→] was attached to the C-3 position of GlcA, and consecutive KS-branched disaccharide sequences were found in a CS chain. KS-branched polysaccharides clearly exhibited resistance to degradation by chondroitinase ABC or ACII (at low concentrations) compared with typical CS structures. Furthermore, KS-branched polysaccharides stimulated neurite outgrowth of hippocampal neurons. These results strongly suggest that M. chinensis is a rich source of KS-branched CS, and it has important biological activities.;
DescriptionBiochemical Journal, 473 4145–4158; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
DepartmentThe Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
PublisherPortland Press Limited
RelationshipsThe Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;
AccessCC BY-NC-ND — Creative Commons Attribution-NonCommercial-NoDerivatives; A full text version is available in DSpace@RPI; Open Access;
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