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dc.rights.licenseCC BY-NC-ND — Creative Commons Attribution-NonCommercial-NoDerivatives
dc.contributor.authorHigashi, K.
dc.contributor.authorTakeda, Keita
dc.contributor.authorMukuno, A.
dc.contributor.authorOkamoto, Y.
dc.contributor.authorMasuko, S.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorToida, T.
dc.identifier.citationIdentification of Keratan Sulfate Disaccharide Unit at the C-3 position of Glucuronic Acid of Chondroitin Sulfate from Mactra chinensis, K. Higashi, K. Takeda, A. Mukuno, Y. Okamoto, S. Masuko, R. J. Linhardt, T. Toida, Biochemical Journal, 473 4145–4158, 2016.
dc.descriptionBiochemical Journal, 473 4145–4158
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractGlycosaminoglycans (GAGs), including chondroitin sulfate (CS), dermatan sulfate, heparin, heparan sulfate and keratan sulfate (KS) are linear sulfated repeating disaccharide sequences containing hexosamine and uronic acid [or galactose (Gal) in the case of KS]. Among the GAGs, CS shows structural variations, such as sulfation patterns and fucosylation, which are responsible for their physiological functions through CS interaction with CS-binding proteins. Here, we solved the structure of KS-branched CS-E derived from a clam, Mactra chinensis. KS disaccharide [d-GlcNAc6S-(1→3)-β-d-Gal-(1→] was attached to the C-3 position of GlcA, and consecutive KS-branched disaccharide sequences were found in a CS chain. KS-branched polysaccharides clearly exhibited resistance to degradation by chondroitinase ABC or ACII (at low concentrations) compared with typical CS structures. Furthermore, KS-branched polysaccharides stimulated neurite outgrowth of hippocampal neurons. These results strongly suggest that M. chinensis is a rich source of KS-branched CS, and it has important biological activities.
dc.publisherPortland Press Limited
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleIdentification of Keratan Sulfate Disaccharide Unit at the C-3 position of Glucuronic Acid of Chondroitin Sulfate from Mactra chinensisen_US
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.accessRightsOpen Access
dc.rights.holderCC BY-NC-ND : this license allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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CC BY-NC-ND — Creative Commons Attribution-NonCommercial-NoDerivatives
Except where otherwise noted, this item's license is described as CC BY-NC-ND — Creative Commons Attribution-NonCommercial-NoDerivatives