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dc.rights.licenseCC BY — Creative Commons Attribution
dc.contributor.authorSordini, L.
dc.contributor.authorGarrudo, F.F.F.
dc.contributor.authorRodrigues, C.A.V.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorCabral, J.M.S.
dc.contributor.authorCastelo Ferreira, F.
dc.contributor.authorMorgado, J.
dc.identifier.citationEffect of electrical stimulation conditions on neural stem cells differentiation on cross-linked PEDOT:PSS films, L. Sordini, F. F. F. Garrudo, C. A. V. Rodrigues, R. J. Linhardt, J. M. S. Cabral, F. Castelo Ferreira, J. Morgado, Frontiers in Bioengineering and Biotechnology, 9, 591838, 2021.
dc.descriptionFrontiers in Bioengineering and Biotechnology, 9, 591838
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractThe ability to culture and differentiate neural stem cells (NSCs) to generate functional neural populations is attracting increasing attention due to its potential to enable cell-therapies to treat neurodegenerative diseases. Recent studies have shown that electrical stimulation improves neuronal differentiation of stem cells populations, highlighting the importance of the development of electroconductive biocompatible materials for NSC culture and differentiation for tissue engineering and regenerative medicine. Here, we report the use of the conjugated polymer poly(3,4-ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS CLEVIOS P AI 4083) for the manufacture of conductive substrates. Two different protocols, using different cross-linkers (3-glycidyloxypropyl)trimethoxysilane (GOPS) and divinyl sulfone (DVS) were tested to enhance their stability in aqueous environments. Both cross-linking treatments influence PEDOT:PSS properties, namely conductivity and contact angle. However, only GOPS-cross-linked films demonstrated to maintain conductivity and thickness during their incubation in water for 15 days. GOPS-cross-linked films were used to culture ReNcell-VM under different electrical stimulation conditions (AC, DC, and pulsed DC electrical fields). The polymeric substrate exhibits adequate physicochemical properties to promote cell adhesion and growth, as assessed by Alamar Blue® assay, both with and without the application of electric fields. NSCs differentiation was studied by immunofluorescence and quantitative real-time polymerase chain reaction. This study demonstrates that the pulsed DC stimulation (1 V/cm for 12 days), is the most efficient at enhancing the differentiation of NSCs into neurons.
dc.description.sponsorshipFundação para a Ciência e Tecnologia
dc.publisherFrontiers Media SA
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofFrontiers in Bioengineering and Biotechnology
dc.rightsAttribution 3.0 United States*
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleEffect of electrical stimulation conditions on neural stem cells differentiation on cross-linked PEDOT: PSS filmsen_US
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.accessRightsOpen Access
dc.rights.holderCC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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