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dc.rights.licenseCC BY — Creative Commons Attribution
dc.contributor.authorAmores de Sousa, Miriam C.
dc.contributor.authorRodrigues, Carlos A.V.
dc.contributor.authorFerreira, Inês A. F.
dc.contributor.authorDiogo, Maria Margarida
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorFerreira, Frederico Castelo
dc.identifier.citationFunctionalization of electrospun nanofibers and fiber alignment enhance neural stem cell proliferation and neuronal differentiation, M. C. Amores de Sousa, C. A. V. Rodrigues, I. A. F. Ferreira, M. Margarida Diogo, R. J. Linhardt, J. M. S. Cabral, F. Castelo Ferreira, Frontiers in Bioengineering and Biotechnology, 8, 580135, 2020.
dc.descriptionFrontiers in Bioengineering and Biotechnology, 8, 580135
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractNeural stem cells (NSCs) have the potential to generate the cells of the nervous system and, when cultured on nanofiber scaffolds, constitute a promising approach for neural tissue engineering. In this work, the impact of combining nanofiber alignment with functionalization of the electrospun poly-ε-caprolactone (PCL) nanofibers with biological adhesion motifs on the culture of an NSC line (CGR8-NS) is evaluated. A five-rank scale for fiber density was introduced, and a 4.5 level, corresponding to 70–80% fiber density, was selected for NSC in vitro culture. Aligned nanofibers directed NSC distribution and, especially in the presence of laminin (PCL-LN) and the RGD-containing peptide GRGDSP (PCL-RGD), promoted higher cell elongation, quantified by the eccentricity and axis ratio. In situ differentiation resulted in relatively higher percentage of cells expressing Tuj1 in PCL-LN, as well as significantly longer neurite development (41.1 ± 1.0 μm) than PCL-RGD (32.0 ± 1.0 μm), pristine PCL (25.1 ± 1.2 μm), or PCL-RGD randomly oriented fibers (26.5 ± 1.4 μm), suggesting that the presence of LN enhances neuronal differentiation. This study demonstrates that aligned nanofibers, functionalized with RGD, perform as well as PCL-LN fibers in terms of cell adhesion and proliferation. The presence of the full LN protein improves neuronal differentiation outcomes, which may be important for the use of this system in tissue engineering applications.
dc.description.sponsorshipNational Institutes of Health
dc.publisherFrontiers Media SA
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofFrontiers in Bioengineering and Biotechnology
dc.rightsAttribution 3.0 United States*
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleFunctionalization of electrospun nanofibers and fiber alignment enhance neural stem cell proliferation and neuronal differentiationen_US
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.accessRightsOpen Access
dc.rights.holderCC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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