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dc.rights.licenseCC BY — Creative Commons Attribution
dc.contributor.authorAwofiranye, Adeola E.
dc.contributor.authorBaytas, Sultan N.
dc.contributor.authorXia, Ke
dc.contributor.authorBadri, Abinaya
dc.contributor.authorHe, Wenqin
dc.contributor.authorVarki, Ajit
dc.contributor.authorKoffas, Mattheos
dc.contributor.authorLinhardt, Robert J.
dc.date2020
dc.date.accessioned2022-06-21T15:36:15Z
dc.date.available2022-06-21T15:36:15Z
dc.date.issued2020
dc.date.issued2020-12-01
dc.identifier.citationN-glycolyl chondroitin synthesis using metabolically engineered E. coli, A. E. Awofiranye, S. N. Baytas, K. Xia, A. Badri, W. He, A. Varki, M. Koffas, R. J. Linhardt, Applied Microbiology and Biotechnology Express,10, 144, 2020.
dc.identifier.issn21910855
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5053
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5053
dc.identifier.urihttps://doi.org/10.1186/s13568-020-01084-6
dc.descriptionApplied Microbiology and Biotechnology Express,10, 144
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractN-glycolyl chondroitin (Gc-CN) is a metabolite of N-glycolylneuraminic acid (Neu5Gc), a sialic acid that is commonly found in mammals, but not humans. Humans can incorporate exogenous Neu5Gc into their tissues from eating red meat. Neu5Gc cannot be biosynthesized by humans due to an evolutionary mutation and has been implicated in causing inflammation causing human diseases, such as cancer. The study Neu5Gc is important in evolutionary biology and the development of potential cancer biomarkers. Unfortunately, there are several limitations to detecting Neu5Gc. The elimination of Neu5Gc involves a degradative pathway leading to the incorporation of N-glycolyl groups into glycosaminoglycans (GAGs), such as Gc-CN. Gc-CN has been found in humans and in animals including mice, lamb and chimpanzees. Here, we present the biosynthesis of Gc-CN in bacteria by feeding chemically synthesized N-glycolylglucosamine to Escherichia coli. A metabolically engineered strain of E. coli K4, fed with glucose supplemented with GlcNGc, converted it to N-glycolylgalactosamine (GalNGc) that could then be utilized as a substrate in the chondroitin biosynthetic pathway. The final product, Gc-CN was converted to disaccharides using chondroitin lyase ABC and analyzed by liquid chromatography–tandem mass spectrometry with multiple reaction monitoring detection. This analysis showed the incorporation of GalNGc into the backbone of the chondroitin oligosaccharide.
dc.description.sponsorshipNational Science Foundation
dc.languageen_US
dc.language.isoENG
dc.publisherSpringer
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofAMB Express
dc.relation.urihttps://harc.rpi.edu/
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleN-glycolyl chondroitin synthesis using metabolically engineered E. colien_US
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.accessRightsOpen Access
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1186/s13568-020-01084-6
dc.rights.holderCC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.volume10


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CC BY — Creative Commons Attribution
Except where otherwise noted, this item's license is described as CC BY — Creative Commons Attribution