Fabrication of homotypic neural ribbons as a multiplex platform optimized for spinal cord delivery
Authors
Olmsted, Zachary T.
Stigliano, Cinzia
Badri, Abinaya
Zhang, Fuming
Williams, Asher
Koffas, Mattheos A.G.
Xie, Yubing
Linhardt, Robert J.
Cibelli, Jose
Horner, Philip J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2020-12-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
Attribution 3.0 United States
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
Full Citation
Fabrication of homotypic neural ribbons as a multiplex platform optimized for spinal cord delivery, Z. T. Olmsted, C. Stigliano, A. Badri, F. Zhang, A. Williams, M. A. G. Koffas, Y. Xie, R. J. Linhardt, J. Cibelli, P. J. Horner, J. L. Paluh, Scientific Reports 10, 12939, 2020
Abstract
Cell therapy for the injured spinal cord will rely on combined advances in human stem cell technologies and delivery strategies. Here we encapsulate homotypic spinal cord neural stem cells (scNSCs) in an alginate-based neural ribbon delivery platform. We perform a comprehensive in vitro analysis and qualitatively demonstrate graft survival and injury site retention using a rat C4 hemi-contusion model. Pre-configured neural ribbons are transport-stable modules that enable site-ready injection, and can support scNSC survival and retention in vivo. Neural ribbons offer multifunctionality in vitro including co-encapsulation of the injury site extracellular matrix modifier chondroitinase ABC (chABC), tested here in glial scar models, and ability of cervically-patterned scNSCs to differentiate within neural ribbons and project axons for integration with 3-D external matrices. This is the first extensive in vitro characterization of neural ribbon technology, and constitutes a plausible method for reproducible delivery, placement, and retention of viable neural cells in vivo.
Description
Scientific Reports 10, 12939
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Nature
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Scientific Reports
https://harc.rpi.edu/
Rensselaer Polytechnic Institute, Troy, NY
Scientific Reports
https://harc.rpi.edu/
Access
Open Access
CC BY — Creative Commons Attribution
CC BY — Creative Commons Attribution