FAM20B-catalyzed glycosaminoglycans control murine tooth number by restricting FGFR2b signaling
AuthorWu, Jingyi; Tian, Ye; Han, Lu; Liu, Chao; Sun, Tianyu; Li, Ling; Yu, Yanlei; Lamichhane, Bikash; D'Souza, Rena N.; Millar, Sarah E.; Krumlauf, Robb; Ornitz, David M.; Feng, Jian Q.; Klein, Ophir; Zhao, Hu; Zhang, Fuming; Linhardt, Robert J.; Wang, Xiaofang
SubjectBiology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Full CitationFAM20B-catalyzed glycosaminoglycans control murine tooth number by restricting FGFR2b signaling, J. Wu, Y. Tian, L. Han, C. Liu, T. Sun, L. Li, Y. Yu, B. Lamichhane, R. N. D’Souza, S. E. Millar, R. Krumlauf, D. M. Ornitz, J. Q. Feng, O. Klein, H. Zhao, F. Zhang, R. J. Linhardt, X. Wang, BMC Biology, 18, 87, 2020.
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AbstractBackground: The formation of supernumerary teeth is an excellent model for studying the molecular mechanisms that control stem/progenitor cell homeostasis needed to generate a renewable source of replacement cells and tissues. Although multiple growth factors and transcriptional factors have been associated with supernumerary tooth formation, the regulatory inputs of extracellular matrix in this regenerative process remains poorly understood. Results: In this study, we present evidence that disrupting glycosaminoglycans (GAGs) in the dental epithelium of mice by inactivating FAM20B, a xylose kinase essential for GAG assembly, leads to supernumerary tooth formation in a pattern reminiscent of replacement teeth. The dental epithelial GAGs confine murine tooth number by restricting the homeostasis of Sox2(+) dental epithelial stem/progenitor cells in a non-autonomous manner. FAM20B-catalyzed GAGs regulate the cell fate of dental lamina by restricting FGFR2b signaling at the initial stage of tooth development to maintain a subtle balance between the renewal and differentiation of Sox2(+) cells. At the later cap stage, WNT signaling functions as a relay cue to facilitate the supernumerary tooth formation. Conclusions: The novel mechanism we have characterized through which GAGs control the tooth number in mice may also be more broadly relevant for potentiating signaling interactions in other tissues during development and tissue homeostasis.;
DescriptionBMC Biology, 18, 87; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
DepartmentThe Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
RelationshipsThe Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; BMC Biology; https://harc.rpi.edu/;
AccessCC BY — Creative Commons Attribution; A full text version is available in DSpace@RPI; Open Access;
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