Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers
Author
Yan, Lufeng; Li, Lingyun; Li, Junhui; Yu, Yanlei; Liu, Xinyue; Ye, Xingqian; Linhardt, Robert J.; Chen, ShiguoOther Contributors
Date Issued
2019-11-01Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineeringDegree
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CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.; Attribution 3.0 United StatesFull Citation
Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers, L. Yan, L. Li, J. Li, Y. Yu, X. Liu, X. Ye, R. J. Linhardt, S. Chen, Glycobiology 29, 755–764, 2019.Metadata
Show full item recordAbstract
Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered. A detailed characterization of FCSs and their derivatives is important to establish their structure–activity relationship in the development of new anticoagulant drugs. In the current study, online hydrophilic interaction chromatography–Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides. These depolymerized FCS fragments were quantified and compared using the glycomics software package, GlycReSoft. The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers. Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method. Thus, a new structural model for FCS chains from these different sea cucumbers was defined. This bottom-up approach provides rich detailed structural analysis and provides quantitative information with high accuracy and reproducibility and should be suitable for the quality control in FCSs as well as their oligosaccharides.;Description
Glycobiology 29, 755–764; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.Department
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);Publisher
Oxford University PressRelationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Glycobiology; https://harc.rpi.edu/;Access
CC BY — Creative Commons Attribution; A full text version is available in DSpace@RPI; Open Access;Collections
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