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dc.rights.licenseCC BY — Creative Commons Attribution
dc.contributor.authorCarvalho, M.S.
dc.contributor.authorSilva, J.C.
dc.contributor.authorUdangawa, R.N.
dc.contributor.authorCabral, J.M.S.
dc.contributor.authorCastelo Ferreira, F.
dc.contributor.authorLobato da Silva, C.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorVashishth, D.
dc.date2019
dc.date.accessioned2022-06-21T16:45:01Z
dc.date.available2022-06-21T16:45:01Z
dc.date.issued2019-06-01
dc.identifier.citationCo-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineering, M.S. Carvalho, J.C. Silva, R.N. Udangawa, J.M. S. Cabral, F. Castelo Ferreira, C. Lobato da Silva, R.J. Linhardt, D. Vashishth, Materials Science and Engineering: C, 99, 479-490, 2019.
dc.identifier.issn18730191
dc.identifier.issn9284931
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5068
dc.identifier.urihttps://doi.org/10.1016/j.msec.2019.01.127
dc.descriptionMaterials Science and Engineering: C, 99, 479-490
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractCell-derived extracellular matrix (ECM) has been employed as scaffolds for tissue engineering, creating a biomimetic microenvironment that provides physical, chemical and mechanical cues for cells and supports cell adhesion, proliferation, migration and differentiation by mimicking their in vivo microenvironment. Despite the enhanced bioactivity of cell-derived ECM, its application as a scaffold to regenerate hard tissues such as bone is still hampered by its insufficient mechanical properties. The combination of cell-derived ECM with synthetic biomaterials might result in an effective strategy to enhance scaffold mechanical properties and structural support. Electrospinning has been used in bone tissue engineering to fabricate fibrous and porous scaffolds, mimicking the hierarchical organized fibrillar structure and architecture found in the ECM. Although the structure of the scaffold might be similar to ECM architecture, most of these electrospun scaffolds have failed to achieve functionality due to a lack of bioactivity and osteoinductive factors. In this study, we developed bioactive cell-derived ECM electrospun polycaprolactone (PCL) scaffolds produced from ECM derived from human mesenchymal stem/stromal cells (MSC), human umbilical vein endothelial cells (HUVEC) and their combination based on the hypothesis that the cell-derived ECM incorporated into the PCL fibers would enhance the biofunctionality of the scaffold. The aims of this study were to fabricate and characterize cell-derived ECM electrospun PCL scaffolds and assess their ability to enhance osteogenic differentiation of MSCs, envisaging bone tissue engineering applications. Our findings demonstrate that all cell-derived ECM electrospun scaffolds promoted significant cell proliferation compared to PCL alone, while presenting similar physical/mechanical properties. Additionally, MSC:HUVEC-ECM electrospun scaffolds significantly enhanced osteogenic differentiation of MSCs as verified by increased ALP activity and osteogenic gene expression levels. To our knowledge, these results describe the first study suggesting that MSC:HUVEC-ECM might be developed as a biomimetic electrospun scaffold for bone tissue engineering applications.
dc.description.sponsorshipNational Science Foundation
dc.languageen_US
dc.language.isoENG
dc.publisherSpringer Nature
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofMaterials Science and Engineering C
dc.relation.urihttps://harc.rpi.edu/
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleCo-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineeringen_US
dc.typeArticle
dcterms.accessRightsOpen Access
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1016/j.msec.2019.01.127
dc.rights.holderCC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages479-490
rpi.description.volume99


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Except where otherwise noted, this item's license is described as CC BY — Creative Commons Attribution