Comparative Genomics Reveals Specific Genetic Architectures in Nicotine Metabolism of Pseudomonas sp. JY-Q
Author
Li, Jun; Qian, Shulan; Xiong, Lie; Zhu, Chengyun; Shu, Ming; Wang, Jie; Jiao, Yang; He, Houlong; Zhang, Fuming; Linhardt, Robert J.; Zhong, WeihongOther Contributors
Date Issued
2017-10-31Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineeringDegree
Terms of Use
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.; Attribution 3.0 United StatesFull Citation
Comparative genomics reveals specific genetic architectures in nicotine metabolism of Pseudomonas sp. JY-Q, J. Li, S. Qian, L. Xiong, C. Zhu, J. Wang, Y. Jiao, H. He, F. Zhang, R. J. Linhardt, W. Zhong, Frontiers in Microbiology, 8, 2085, 2017.Metadata
Show full item recordAbstract
Microbial degradation of nicotine is an important process to control nicotine residues in the aqueous environment. In this study, a high active nicotine degradation strain named Pseudomonas sp. JY-Q was isolated from tobacco waste extract (TWE). This strain could completely degrade 5.0 g l−1 nicotine in 24 h under optimal culture conditions, and it showed some tolerance even at higher concentrations (10.0 g l−1) of nicotine. The complete genome of JY-Q was sequenced to understand the mechanism by which JY-Q could degrade nicotine and tolerate such high nicotine concentrations. Comparative genomic analysis indicated that JY-Q degrades nicotine through putative novel mechanisms. Two candidate gene cluster duplications located separately at distant loci were predicted to be responsible for nicotine degradation. These two nicotine (Nic) degradation-related loci (AA098_21325—AA098_21340, AA098_03885—AA098_03900) exhibit nearly completely consistent gene organization and component synteny. The nicotinic acid (NA) degradation gene cluster (AA098_17770–AA098_17790) and Nic-like clusters were both predicted to be flanked by mobile genetic elements (MGE). Furthermore, we analyzed the regions of genomic plasticity (RGP) in the JY-Q strain and found a dynamic genome carrying a type VI secretion system (T6SS) that promotes nicotine metabolism and tolerance based on transcriptomics and used in silico methods to identify the T6SS effector protein. Thus, a novel nicotine degradation mechanism was elucidated for Pseudomonas sp. JY-Q, suggesting its potential application in the bioremediation of nicotine-contaminated environments, such as TWEs.;Description
Comparative genomics reveals specific genetic architectures in nicotine metabolism of Pseudomonas sp. JY-QDepartment
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);Publisher
Frontiers Media SARelationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Frontiers in Microbiology; https://harc.rpi.edu/;Access
CC BY — Creative Commons Attribution; Open Access; A full text version is available in DSpace@RPI;Collections
The following license files are associated with this item: