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dc.rights.licenseCC BY — Creative Commons Attribution
dc.contributor.authorLi, Jun
dc.contributor.authorQian, Shulan
dc.contributor.authorXiong, Lie
dc.contributor.authorZhu, Chengyun
dc.contributor.authorShu, Ming
dc.contributor.authorWang, Jie
dc.contributor.authorJiao, Yang
dc.contributor.authorHe, Houlong
dc.contributor.authorZhang, Fuming
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorZhong, Weihong
dc.date2017
dc.date.accessioned2022-06-21T17:20:29Z
dc.date.available2022-06-21T17:20:29Z
dc.date.issued2017-10-31
dc.identifier.citationComparative genomics reveals specific genetic architectures in nicotine metabolism of Pseudomonas sp. JY-Q, J. Li, S. Qian, L. Xiong, C. Zhu, J. Wang, Y. Jiao, H. He, F. Zhang, R. J. Linhardt, W. Zhong, Frontiers in Microbiology, 8, 2085, 2017.
dc.identifier.issn1664302X
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5078
dc.identifier.urihttps://doi.org/10.3389/fmicb.2017.02085
dc.descriptionComparative genomics reveals specific genetic architectures in nicotine metabolism of Pseudomonas sp. JY-Q
dc.description.abstractMicrobial degradation of nicotine is an important process to control nicotine residues in the aqueous environment. In this study, a high active nicotine degradation strain named Pseudomonas sp. JY-Q was isolated from tobacco waste extract (TWE). This strain could completely degrade 5.0 g l−1 nicotine in 24 h under optimal culture conditions, and it showed some tolerance even at higher concentrations (10.0 g l−1) of nicotine. The complete genome of JY-Q was sequenced to understand the mechanism by which JY-Q could degrade nicotine and tolerate such high nicotine concentrations. Comparative genomic analysis indicated that JY-Q degrades nicotine through putative novel mechanisms. Two candidate gene cluster duplications located separately at distant loci were predicted to be responsible for nicotine degradation. These two nicotine (Nic) degradation-related loci (AA098_21325—AA098_21340, AA098_03885—AA098_03900) exhibit nearly completely consistent gene organization and component synteny. The nicotinic acid (NA) degradation gene cluster (AA098_17770–AA098_17790) and Nic-like clusters were both predicted to be flanked by mobile genetic elements (MGE). Furthermore, we analyzed the regions of genomic plasticity (RGP) in the JY-Q strain and found a dynamic genome carrying a type VI secretion system (T6SS) that promotes nicotine metabolism and tolerance based on transcriptomics and used in silico methods to identify the T6SS effector protein. Thus, a novel nicotine degradation mechanism was elucidated for Pseudomonas sp. JY-Q, suggesting its potential application in the bioremediation of nicotine-contaminated environments, such as TWEs.
dc.description.sponsorshipNational Natural Science Foundation of China
dc.languageen_US
dc.language.isoENG
dc.publisherFrontiers Media SA
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofFrontiers in Microbiology
dc.relation.urihttps://harc.rpi.edu/
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleComparative Genomics Reveals Specific Genetic Architectures in Nicotine Metabolism of Pseudomonas sp. JY-Qen_US
dc.typeArticle
dcterms.accessRightsOpen Access
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.3389/fmicb.2017.02085
dc.rights.holderCC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.volume8


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Except where otherwise noted, this item's license is described as CC BY — Creative Commons Attribution