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dc.rights.licenseCC BY — Creative Commons Attribution
dc.contributor.authorAndrew Jones, J.
dc.contributor.authorVernacchio, Victoria R.
dc.contributor.authorCollins, Shannon M.
dc.contributor.authorShirke, Abhijit N.
dc.contributor.authorXiu, Yu
dc.contributor.authorEnglaender, Jacob A.
dc.contributor.authorCress, Brady F.
dc.contributor.authorMcCutcheon, Catherine C.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorGross, Richard A.
dc.contributor.authorKoffas, Mattheos A.G.
dc.identifier.citationComplete biosynthesis of anthocyanins using E. coli polycultures J. Jones, V. Vernacchio, S. Collins, A.Shirke, Y. Xiu, J. Englaender, B, Cress, C. McCutcheon, R. J. Linhradt, R. Gross, and M. Koffas, mBio, 8, e00621-17, 2017.
dc.descriptionComplete biosynthesis of anthocyanins using E. coli polycultures
dc.description.abstractFermentation-based chemical production strategies provide a feasible route for the rapid, safe, and sustainable production of a wide variety of important chemical products, ranging from fuels to pharmaceuticals. These strategies have yet to find wide industrial utilization due to their inability to economically compete with traditional extraction and chemical production methods. Here, we engineer for the first time the complex microbial biosynthesis of an anthocyanin plant natural product, starting from sugar. This was accomplished through the development of a synthetic, 4-strain Escherichia coli polyculture collectively expressing 15 exogenous or modified pathway enzymes from diverse plants and other microbes. This synthetic consortium-based approach enables the functional expression and connection of lengthy pathways while effectively managing the accompanying metabolic burden. The de novo production of specific anthocyanin molecules, such as calistephin, has been an elusive metabolic engineering target for over a decade. The utilization of our polyculture strategy affords milligram-per-liter production titers. This study also lays the groundwork for significant advances in strain and process design toward the development of cost-competitive biochemical production hosts through nontraditional methodologies.
dc.description.sponsorshipNational Science Foundation
dc.publisherAmerican Society for Microbiology (ASM)
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.rightsAttribution 3.0 United States*
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleComplete biosynthesis of anthocyanins using E. coli polyculturesen_US
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.accessRightsOpen Access
dc.rights.holderCC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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