Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process
Author
Zhi, Zijian; Chen, Jianle; Li, Shan; Wang, Wenjun; Huang, Rui; Liu, Donghong; DIng, Tian; Linhardt, Robert J. John; Chen, Shiguo; Ye, XingqianOther Contributors
Date Issued
2017-12-01Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineeringDegree
Terms of Use
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.; Attribution 3.0 United StatesFull Citation
Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process, Z. Zhi, J. Chen, S. Li, W. Wang, R. Huang, D. Liu, Tian Ding, R. J. Linhardt, S. Chen, X. Ye, Scientific Reports, 7, 541, 2017.Metadata
Show full item recordAbstract
Pectin, a natural polysaccharide found in the cell wall of most higher plant such as citrus, has drawn much attention due to its potential beneficial role in facilitating the treatment of many diseases like cancer, hyper cholesterol and diabetes. However, the broad application of pectin faces great limitations as the large molecular size of pectin severely prevents its bioavailability in vivo. In this study, we report an effective and highly convenient approach to degrade natural pectin into lower molecular pectin. By combining ultrasound with Fenton system (US-Fenton), we show that ultrasound synergistically enhances the efficiency of Fenton reaction to degrade pectin into 5.5 kDa within only 35 minutes. Importantly, RG-I domain, the most effective portion of natural pectin, was well preserved and highly enriched. In addition, the antioxidant activities of US-Fenton-treated pectin was significantly elevated. The mechanism of this novel observation was further investigated through the multiple structural analyses including HPLC, IR and NMR. Taken together, we present a novel and convenient approach to generate ultra-low molecular weight pectin with high efficiency and higher bioactivity. We expect our approach will have broader applications in improving the bioavailability and bioactivity of other polysaccharide-based natural compounds.;Description
Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton processDepartment
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);Publisher
NatureRelationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Scientific Reports; https://harc.rpi.edu/;Access
CC BY — Creative Commons Attribution; A full text version is available in DSpace@RPI; Open Access;Collections
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