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dc.rights.licenseCC BY — Creative Commons Attribution
dc.contributor.authorSchultz, Victor
dc.contributor.authorSuflita, Mathew
dc.contributor.authorLiu, Xinyue
dc.contributor.authorZhang, Xing
dc.contributor.authorYu, Yanlei
dc.contributor.authorLi, Lingyun
dc.contributor.authorGreen, Dixy E.
dc.contributor.authorXu, Yongmei
dc.contributor.authorZhang, Fuming
dc.contributor.authorDeAngelis, Paul L.
dc.contributor.authorLiu, Jian
dc.contributor.authorLinhardt, Robert J.
dc.identifier.citationHeparan Sulfate Domains Required for Fibroblast Growth Factor 1 and 2 Signaling through Fibroblast Growth Factor Receptor 1c, V. Schultz, M. Suflita, X. Liu, X. Zhang, Y. Yu, L. Li, D. E. Green, Y. Xu, F. Zhang, P. L. DeAngelis, J. Liu, R. J. Linhardt, Journal of Biological Chemistry, 292, 2495–2509, 2017.
dc.descriptionHeparan Sulfate Domains Required for Fibroblast Growth Factor 1 and 2 Signaling through Fibroblast Growth Factor Receptor 1c
dc.description.abstractA small library of well defined heparan sulfate (HS) polysaccharides was chemoenzymatically synthesized and used for a detailed structure-activity study of fibroblast growth factor (FGF) 1 and FGF2 signaling through FGF receptor (FGFR) 1c. The HS polysaccharide tested contained both undersulfated (NA) domains and highly sulfated (NS) domains as well as very well defined non-reducing termini. This study examines differences in the HS selectivity of the positive canyons of the FGF12-FGFR1c2 and FGF22-FGFR1c2 HS binding sites of the symmetric FGF2-FGFR2-HS2 signal transduction complex. The results suggest that FGF12-FGFR1c2 binding site prefers a longer NS domain at the non-reducing terminus than FGF22-FGFR1c2 In addition, FGF22-FGFR1c2 can tolerate an HS chain having an N-acetylglucosamine residue at its non-reducing end. These results clearly demonstrate the different specificity of FGF12-FGFR1c2 and FGF22-FGFR1c2 for well defined HS structures and suggest that it is now possible to chemoenzymatically synthesize precise HS polysaccharides that can selectively mediate growth factor signaling. These HS polysaccharides might be useful in both understanding and controlling the growth, proliferation, and differentiation of cells in stem cell therapies, wound healing, and the treatment of cancer.
dc.description.sponsorshipNational Institutes of Health
dc.publisherThe American Society for Biochemistry and Molecular Biology (ASBMB) and Elsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofJournal of Biological Chemistry
dc.rightsAttribution 3.0 United States*
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleHeparan Sulfate Domains Required for Fibroblast Growth Factor 1 and 2 Signaling through Fibroblast Growth Factor Receptor 1cen_US
dcterms.accessRightsOpen Access
dcterms.accessRightsA full text version is available in DSpace@RPI
dc.rights.holderCC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)

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