CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production
AuthorCress, Brady F.; Leitz, Quentin D.; Kim, Daniel C.; Amore, Teresita D.; Suzuki, Jon Y.; Linhardt, Robert J.; Koffas, Mattheos A.G.
SubjectBiology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
Full CitationCRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production, B. F. Cress, Q. D. Leitz, D. C. Kim, T. D. Amore, J. Y. Suzuki, R. J. Linhardt, Mattheos A. G. Koffas, Microbial Cell Factories, 16, (10), 1-15, 2017.
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AbstractAnthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. Although all anthocyanins exhibit pH-responsive photochemical changes, distinct structural decorations on the core anthocyanin skeleton also cause dramatic color shifts, in addition to improved stabilities and unique pharmacological properties. In this work, we report for the first time the extension of the reconstituted plant anthocyanin pathway from (+)-catechin to O-methylated anthocyanins in a microbial production system, an effort which requires simultaneous co-option of the endogenous metabolites UDP-glucose and S-adenosyl-L-methionine (SAM or AdoMet).;
DescriptionCRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production
DepartmentThe Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
RelationshipsThe Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Microbial Cell Factories; https://harc.rpi.edu/;
AccessCC BY — Creative Commons Attribution; Open Access; A full text version is available in DSpace@RPI;
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