CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production
Author
Cress, Brady F.; Leitz, Quentin D.; Kim, Daniel C.; Amore, Teresita D.; Suzuki, Jon Y.; Linhardt, Robert J.; Koffas, Mattheos A.G.Other Contributors
Date Issued
2017-01-17Subject
Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineeringDegree
Terms of Use
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.; Attribution 3.0 United StatesFull Citation
CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin production, B. F. Cress, Q. D. Leitz, D. C. Kim, T. D. Amore, J. Y. Suzuki, R. J. Linhardt, Mattheos A. G. Koffas, Microbial Cell Factories, 16, (10), 1-15, 2017.Metadata
Show full item recordAbstract
Anthocyanins are a class of brightly colored, glycosylated flavonoid pigments that imbue their flower and fruit host tissues with hues of predominantly red, orange, purple, and blue. Although all anthocyanins exhibit pH-responsive photochemical changes, distinct structural decorations on the core anthocyanin skeleton also cause dramatic color shifts, in addition to improved stabilities and unique pharmacological properties. In this work, we report for the first time the extension of the reconstituted plant anthocyanin pathway from (+)-catechin to O-methylated anthocyanins in a microbial production system, an effort which requires simultaneous co-option of the endogenous metabolites UDP-glucose and S-adenosyl-L-methionine (SAM or AdoMet).;Description
CRISPRi-mediated metabolic engineering of E. coli for O-methylated anthocyanin productionDepartment
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);Publisher
Springer NatureRelationships
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Microbial Cell Factories; https://harc.rpi.edu/;Access
CC BY — Creative Commons Attribution; Open Access; A full text version is available in DSpace@RPI;Collections
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