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dc.rights.licenseCC BY — Creative Commons Attribution
dc.contributor.authorMeli, Luciana
dc.contributor.authorBarbosa, Hélder S.C.
dc.contributor.authorHickey, Anne Marie
dc.contributor.authorGasimli, Leyla
dc.contributor.authorNierode, Gregory
dc.contributor.authorDiogo, Maria Margarida
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorDordick, Jonathan S.
dc.date2014
dc.date.accessioned2022-06-21T18:37:21Z
dc.date.available2022-06-21T18:37:21Z
dc.date.issued2014-01-01
dc.identifier.citationThree Dimensional Cellular Microarray Platform for Human Neural Stem Cell Differentiation and Toxicology, L. Meli, H. S.C. Barbosa, A. M. Hickey, L. Gasimli, G. Nierode, M. M. Diogo, R. J. Linhardt, J.M.S. Cabral, Jonathan S. Dordick, Stem Cell Research, 12, 36–47, 2014.
dc.identifier.issn18767753
dc.identifier.issn18735061
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5098
dc.identifier.urihttps://doi.org/10.1016/j.scr.2014.04.004
dc.descriptionStem Cell Research, 12, 36–47
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractWe developed a three-dimensional (3D) cellular microarray platform for the high-throughput (HT) analysis of human neural stem cell (hNSC) growth and differentiation. The growth of an immortalized hNSC line, ReNcell VM, was evaluated on a miniaturized cell culture chip consisting of 60nl spots of cells encapsulated in alginate, and compared to standard 2D well plate culture conditions. Using a live/dead cell viability assay, we demonstrated that the hNSCs are able to expand on-chip, albeit with lower proliferation rates and viabilities than in conventional 2D culture platforms. Using an in-cell, on-chip immunofluorescence assay, which provides quantitative information on cellular levels of proteins involved in neural fate, we demonstrated that ReNcell VM can preserve its multipotent state during on-chip expansion. Moreover, differentiation of the hNSCs into glial progeny was achieved both off- and on-chip six days after growth factor removal, accompanied by a decrease in the neural progenitor markers. The versatility of the platform was further demonstrated by complementing the cell culture chip with a chamber system that allowed us to screen for differential toxicity of small molecules to hNSCs. Using this approach, we showed differential toxicity when evaluating three neurotoxic compounds and one antiproliferative compound, and the null effect of a non-toxic compound at relevant concentrations. Thus, our 3D high-throughput microarray platform may help predict, in vitro, which compounds pose an increased threat to neural development and should therefore be prioritized for further screening and evaluation.
dc.description.sponsorshipNational Institutes of Health
dc.languageen_US
dc.language.isoENG
dc.publisherElsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofStem Cell Research
dc.relation.urihttps://harc.rpi.edu/
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleThree Dimensional Cellular Microarray Platform for Human Neural Stem Cell Differentiation and Toxicologyen_US
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.accessRightsOpen Access
dcterms.isPartOfJournal
dcterms.isVersionOfhttps://doi.org/10.1016/j.scr.2014.04.004
dc.rights.holderCC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
rpi.description.pages36-47
rpi.description.volume13


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CC BY — Creative Commons Attribution
Except where otherwise noted, this item's license is described as CC BY — Creative Commons Attribution