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    Preparation of hyaluronan O-methylester and its enzymatic degradation

    Author
    Hirano, K.; Sakai, Shinobu; Ishikawa, T.; Avci, F.Y.; Linhardt, Robert J.; Toida, T.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    PREPARATION OF THE METHYL ESTER OF HYALURONAN AND ITS ENZYMATIC.pdf (388.5Kb)
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    Date Issued
    2005
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Preparation of hyaluronan O-methylester and its enzymatic degradation, K. Hirano , S. Sakai, T. Ishikawa, F.Y. Avci, R. J. Linhardt, T. Toida, Carbohydrate Research, 340, 2297-2304, 2005.
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    URI
    https://hdl.handle.net/20.500.13015/5151; https://doi.org/10.1016%2Fj.carres.2005.07.016
    Abstract
    A methyl ester of hyaluronan in which the carboxyl groups were fully esterified was prepared using trimethylsilyl diazomethane. This derivative, while not depolymerized by hyaluronan lyases or hyaluronan hydrolases, was a substrate for both chondroitin ACI lyase (EC 4.2.2.5) from Flavobacterium heparinum and chondroitin ACII lyase (EC 4.2.2.5) from Arthrobacter aurescens. The major product isolated in these depolymerization reactions was methyl α-l-threo-hex-4-enepyranosyluronate-(1→3)-2-acetamido-2-deoxy-α,β-d-glucopyranoside as determined by 1H NMR spectroscopy and MALDITOF mass spectrometry.;
    Description
    Carbohydrate Research, 340, 2297-2304; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Elsevier
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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