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dc.contributor.authorHirano, K.
dc.contributor.authorSakai, Shinobu
dc.contributor.authorIshikawa, T.
dc.contributor.authorAvci, F.Y.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorToida, T.
dc.date2005
dc.date.accessioned2022-06-23T03:52:47Z
dc.date.available2022-06-23T03:52:47Z
dc.date.issued2005
dc.identifier.citationPreparation of hyaluronan O-methylester and its enzymatic degradation, K. Hirano , S. Sakai, T. Ishikawa, F.Y. Avci, R. J. Linhardt, T. Toida, Carbohydrate Research, 340, 2297-2304, 2005.
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5151
dc.identifier.urihttps://doi.org/10.1016%2Fj.carres.2005.07.016
dc.descriptionCarbohydrate Research, 340, 2297-2304
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractA methyl ester of hyaluronan in which the carboxyl groups were fully esterified was prepared using trimethylsilyl diazomethane. This derivative, while not depolymerized by hyaluronan lyases or hyaluronan hydrolases, was a substrate for both chondroitin ACI lyase (EC 4.2.2.5) from Flavobacterium heparinum and chondroitin ACII lyase (EC 4.2.2.5) from Arthrobacter aurescens. The major product isolated in these depolymerization reactions was methyl α-l-threo-hex-4-enepyranosyluronate-(1→3)-2-acetamido-2-deoxy-α,β-d-glucopyranoside as determined by 1H NMR spectroscopy and MALDITOF mass spectrometry.
dc.languageen_US
dc.language.isoENG
dc.publisherElsevier
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titlePreparation of hyaluronan O-methylester and its enzymatic degradation
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isVersionOfhttps://doi.org/10.1016%2Fj.carres.2005.07.016
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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