Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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Capillary Electrophoresis for the Analysis of Glycosaminoglycans and Glycosaminoglycan-Derived Oligosaccharides, W.J. Mao, C. Thanawiroon, R.J. Linhardt, Biomedical Chromatography, 16, 77-94, 2002.
Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its high resolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupled to laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detection at 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive amination, which is one of the most important method of derivatization of disaccharides for CE-LIF detection. Outlined in this chapter is a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.;
Biomedical Chromatography, 16, 77-94; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biomedical Chromatography; https://harc.rpi.edu/;