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    Capillary Electrophoresis for the Analysis of Glycosaminoglycans and Glycosaminoglycan-Derived Oligosaccharides

    Author
    Mao, W.J.; Thanawiroon, C.; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    CAPILLARY ELECTROPHORESIS FOR THE ANALYSIS OF.pdf (264.3Kb)
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    Date Issued
    2002-03-20
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Capillary Electrophoresis for the Analysis of Glycosaminoglycans and Glycosaminoglycan-Derived Oligosaccharides, W.J. Mao, C. Thanawiroon, R.J. Linhardt, Biomedical Chromatography, 16, 77-94, 2002.
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    URI
    https://hdl.handle.net/20.500.13015/5160; https://doi.org/10.1002/bmc.153
    Abstract
    Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its high resolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupled to laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detection at 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive amination, which is one of the most important method of derivatization of disaccharides for CE-LIF detection. Outlined in this chapter is a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.;
    Description
    Biomedical Chromatography, 16, 77-94; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Wiley
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biomedical Chromatography; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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