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    Preparation and Structural Determination of Large Oligosaccharides Derived from Acharan Sulfate

    Author
    Chi, Lianli; Munoz, Eva M.; Choi, Hyung Seok; Ha, Young Wan; Kim, Yeong Shik; Toida, Toshihiko; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    PREPARATION AND STRUCTURAL DETERMINATION OF LARGE.pdf (1.307Mb)
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    Date Issued
    2006-05-22
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Preparation and Structural Determination of Large Oligosaccharides Derived from Acharan Sulfate, L. Chi, E. M. Munoz, H. S. Choi, Y. W. Ha, Y. S. Kim, T. Toida, R. J. Linhardt, Carbohydrate Research, 341 864–869, 2006.
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    URI
    https://hdl.handle.net/20.500.13015/5176; https://doi.org/10.1016/j.carres.2006.02.030
    Abstract
    The structures of a series of large oligosaccharides derived from acharan sulfate were characterized. Acharan sulfate is an unusual glycosaminoglycan isolated from the giant African snail, Achatina fulica. Oligosaccharides from decasaccharide to hexadecasaccharide were enzymatically prepared using heparin lyase II and purified. Capillary electrophoresis and gel electrophoresis confirmed the purity of these oligosaccharides. Their structures, determined by ESI-MS and NMR, were consistent with the major repeating sequence in acharan sulfate, -->4)-alpha-d-GlcN(p)Ac-(1-->4)-alpha-l-IdoA(p)2S-(1-->, terminated by 4-linked alpha-d-GlcN(p)Ac residue at the reducing end and by 4,5-unsaturated pyranosyluronic acid 2-sulfate at the non-reducing end.;
    Description
    Carbohydrate Research, 341, 864–869; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Elsevier
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Carbohydrate Research; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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