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dc.contributor.authorCopeland, R.
dc.contributor.authorBalasubramaniam, A.
dc.contributor.authorTiwari, Vaibhav
dc.contributor.authorZhang, F.
dc.contributor.authorBridges, A.
dc.contributor.authorLinhardt, Robert J.
dc.contributor.authorShukla, D.
dc.contributor.authorLiu, J.
dc.date2008
dc.date.accessioned2022-06-23T03:58:45Z
dc.date.available2022-06-23T03:58:45Z
dc.date.issued2008
dc.identifier.citationUsing a 3-O-Sulfated Heparan Sulfate Octasaccharide to Inhibit the Entry of Herpes Simplex Virus 1, R. Copeland, A. Balasubramaniam, V. Tiwari, F. Zhang, A. Bridges, R. J. Linhardt, D. Shukla, J. Liu, Biochemistry, 47, 5774–5783, 2008.
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5191
dc.identifier.urihttps://doi.org/10.1021%2Fbi800205t
dc.descriptionBiochemistry, 47, 5774–5783
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparan sulfate (HS) is a highly sulfated polysaccharide and is present in large quantities on the cell surface and in the extracellular matrix. Herpes simplex virus type 1(HSV-1) utilizes a specialized cell surface HS, known as 3-O-sulfated HS, as an entry receptor to establish infection. Here, we exploit an approach to inhibit HSV-1 infection by using a 3-O-sulfated octasaccharide, mimicking the active domain of the entry receptor. The 3-O-sulfated octasaccharide was synthesized by incubating a heparin octasaccharide (3-OH octasaccharide) with HS 3-O-sulfotransferase isoform 3. The resultant 3-O-sulfated octasaccharide has a structure of ΔUA2S-GlcNS6S-IdoUA2S-GlcNS6S-IdoUA2S-GlcNS3S6S-IdoUA2S-GlcNS6S (where ΔUA is 4-deoxy-α-l-threo-hex-4-enopyranosyluronic acid, GlcN is d-glucosamine and IdoUA is l-iduronic acid). Results from cell based assays revealed that the 3-O-sulfated octasaccharide has stronger activity in blocking HSV-1 infection than that of the 3-OH octasaccharide, suggesting that the inhibition of HSV-1 infection requires a unique sulfation moiety. Our results suggest the feasibility of inhibiting HSV-1 infection by blocking viral entry with a specific oligosaccharide.
dc.languageen_US
dc.language.isoENG
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleUsing a 3-O-Sulfated Heparan Sulfate Octasaccharide to Inhibit the Entry of Herpes Simplex Virus 1
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isVersionOfhttps://doi.org/10.1021%2Fbi800205t
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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