Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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Sulfonation of papain treated chitosan and its mechanism for anticoagulant activity, J. Suwan, Z. Zhang, B. Li, P. Vongchan, P. Meepowpan, F. Zhang, S.A. Mousa, S. Mousa, B. Premanode, P. Kongtawelert, R. J. Linhardt, Carbohydrate Research, 344, 1190–1196, 2009.
The novel low molecular weight chitosan polysulfate (MW 5,120–26,200 Da) was prepared using the depolymerization of chitosan with papain (EC. 22.214.171.124). The sulfonation of depolymerized products was performed using chlorosulfonic acid in N, N-dimethylformamide under semi-heterogeneous conditions. The structures of the products were characterized by FTIR, 13C NMR, and 1H NMR (1D, 2D NMR) spectroscopy. The present study sheds light on the mechanism of anticoagulant activity of chitosan polysulfate. Anticoagulant activity was investigated by an activated partial thromboplastin assay, a thrombin time assay, a prothrombin time assay and thrombelastography. Surface plasmon resonance also provided valuable data for understanding the relationship between the molecular binding of sulfated chitosan to two important blood clotting regulators, antithrombin III and heparin cofactor II. These results show that the principal mechanism by which this chitosan polysulfate exhibits anticoagulant activity is mediated through heparin cofactor II and is dependent on polysaccharide molecular weight.;
Carbohydrate Research, 344, 1190–1196; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Carbohydrate Research; https://harc.rpi.edu/;