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    Variation of Acharan Sulfate and Monosaccharide Composition and Analysis of Neutral N-Glycans in African Giant Snail (Acatina fulica)

    Author
    Park, Y.; Zhang, Z.; Laremore, T.N.; Li, B.; Sim, J.S.; Im, A.R.; Ahn, M.Y.; Kim, Y.S.; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    VARIATION OF ACHARAN SULFATE AND MONOSACCHARIDE COMPOSITION.pdf (1.160Mb)
    Other Contributors
    Date Issued
    2008
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Variation of Acharan Sulfate and Monosaccharide Composition and Analysis of Neutral N-Glycans in African Giant Snail (Acatina fulica). Y. Park, Z. Zhang, T. N. Laremore, B. Li, J.-S. Sim, A.-R. Im, M. Y. Ahn, Y. S. Kim, R.J. Linhardt Glycoconjugate Journal, 25, 863-877, 2008.
    Metadata
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    URI
    https://hdl.handle.net/20.500.13015/5227; https://doi.org/10.1007/s10719-008-9149-1
    Abstract
    Acharan sulfate content from African giant snail (Achatina fulica) was compared in eggs and snails of different ages. Acharan sulfate was not found in egg. Acharan sulfate disaccharide -->4)-alpha-D-GlcNpAc (1-->4)-alpha-L-IdoAp2S(1-->, analyzed by SAX (strong-anion exchange)-HPLC was observed soon after hatching and increases as the snails grow. Monosaccharide compositional analysis showed that mole % of glucosamine, a major monosaccharide of acharan sulfate, increased with age while mole % of galactose decreased with age. These results suggest that galactans represent a major energy source during development, while acharan sulfate appearing immediately after hatching, is essential for the snail growth. The structures of neutral N-glycans released from eggs by peptide N-glycosidase F (PNGase F), were next elucidated using ESI-MS/MS, MALDI-MS/MS, enzyme digestion, and monosaccharide composition analysis. Three types of neutral N-glycan structures were observed, truncated (Hex(2-4)-HexNAc(2)), high mannose (Hex(5-9)-HexNAc(2)), and complex (Hex(3)-HexNAc(2-10)) types. None showed core fucosylation.;
    Description
    Glycoconjugate Journal, 25, 863-877; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Springer
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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