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dc.contributor.authorPark, Y.
dc.contributor.authorZhang, Z.
dc.contributor.authorLaremore, T.N.
dc.contributor.authorLi, B.
dc.contributor.authorSim, J.S.
dc.contributor.authorIm, A.R.
dc.contributor.authorAhn, M.Y.
dc.contributor.authorKim, Y.S.
dc.contributor.authorLinhardt, Robert J.
dc.date2008
dc.date.accessioned2022-06-23T04:01:54Z
dc.date.available2022-06-23T04:01:54Z
dc.date.issued2008
dc.identifier.citationVariation of Acharan Sulfate and Monosaccharide Composition and Analysis of Neutral N-Glycans in African Giant Snail (Acatina fulica). Y. Park, Z. Zhang, T. N. Laremore, B. Li, J.-S. Sim, A.-R. Im, M. Y. Ahn, Y. S. Kim, R.J. Linhardt Glycoconjugate Journal, 25, 863-877, 2008.
dc.identifier.urihttps://hdl.handle.net/20.500.13015/5227
dc.identifier.urihttps://doi.org/10.1007/s10719-008-9149-1
dc.descriptionGlycoconjugate Journal, 25, 863-877
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractAcharan sulfate content from African giant snail (Achatina fulica) was compared in eggs and snails of different ages. Acharan sulfate was not found in egg. Acharan sulfate disaccharide -->4)-alpha-D-GlcNpAc (1-->4)-alpha-L-IdoAp2S(1-->, analyzed by SAX (strong-anion exchange)-HPLC was observed soon after hatching and increases as the snails grow. Monosaccharide compositional analysis showed that mole % of glucosamine, a major monosaccharide of acharan sulfate, increased with age while mole % of galactose decreased with age. These results suggest that galactans represent a major energy source during development, while acharan sulfate appearing immediately after hatching, is essential for the snail growth. The structures of neutral N-glycans released from eggs by peptide N-glycosidase F (PNGase F), were next elucidated using ESI-MS/MS, MALDI-MS/MS, enzyme digestion, and monosaccharide composition analysis. Three types of neutral N-glycan structures were observed, truncated (Hex(2-4)-HexNAc(2)), high mannose (Hex(5-9)-HexNAc(2)), and complex (Hex(3)-HexNAc(2-10)) types. None showed core fucosylation.
dc.languageen_US
dc.language.isoENG
dc.publisherSpringer
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.urihttps://harc.rpi.edu/
dc.subjectBiology
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleVariation of Acharan Sulfate and Monosaccharide Composition and Analysis of Neutral N-Glycans in African Giant Snail (Acatina fulica)
dc.typeArticle
dcterms.accessRightsA full text version is available in DSpace@RPI
dcterms.isVersionOfhttps://doi.org/10.1007/s10719-008-9149-1
dc.rights.holderIn Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/
dc.creator.identifierhttps://orcid.org/0000-0003-2219-5833
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)


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