Evaluation of the Experimental Parameters which Control Electron Detachment Dissociation, and their Effect on the Fragmentation Efficiency of Glycosaminolgycan Carbohydrates
Authors
Leach III, Franklin E.
Wolff, J.J.
Laremore, T.N.
Linhardt, Robert J.
Amster, I.J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2008
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
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Full Citation
Evaluation of the Experimental Parameters which Control Electron Detachment Dissociation, and their Effect on the Fragmentation Efficiency of Glycosaminolgycan Carbohydrates, F. E. Leach III, J. J. Wolff, T. N. Laremore, R. J. Linhardt, I. J. Amster, International Journal of Mass Spectrometry, 276,110-115, 2008.
Abstract
The efficiency of conversion of precursor ions to observable products for electron detachment dissociation (EDD) was measured as a function of the key experimental parameters to determine their optimal values for the Fourier transform mass spectrometry analysis of anionic glycosaminoglycan carbohydrates. These parameters include electron current, electron energy, dispenser cathode heater current, electron beam duration, charge state of the precursor ion, oligomer length, and precursor ion number accumulated in an external radio frequency multipole trap. Precursor conversion is most efficient at an electron current of 15 µA, and decreases at higher and lower values. The conversion of precursor to product ions increases in efficiency as the electron pulse duration is increased. Together, these data suggest that a radially repulsive electric field is produced between the electron beam and negative ions during EDD which causes the reaction cross section to decrease at higher values of electron current (>15 µA). Elevating the heater current of the dispenser cathode increases the electron flux, but also causes ion activation, presumably by blackbody infrared irradiation. An electronic circuit is described that allows the electron current produced by the dispenser cathode to be measured during an EDD or electron capture dissociation (ECD) experiment.
Description
International Journal of Mass Spectrometry, 276,110-115
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Elsevier
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
https://harc.rpi.edu/
Rensselaer Polytechnic Institute, Troy, NY
https://harc.rpi.edu/
Access
A full text version is available in DSpace@RPI