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    Modification of solid phase red cell adherence assay for the detection of platelet alloantibodies in patients with thrombocytopenia

    Author
    Vongchan, P.; Nawarawong, W.; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    MODIFICATION OF SOLID PHASE RED CELL ADHERENCE ASSAY FOR THE.pdf (2.197Mb)
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    Date Issued
    2008
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Modification of solid phase red cell adherence assay for the detection of platelet alloantibodies in patients with thrombocytopenia, P. Vongchan, W. Nawarawong, R. J. Linhardt, American Journal of Clinical Pathology, 130, 455-66, 2008.
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    URI
    https://hdl.handle.net/20.500.13015/5234; https://doi.org/10.1309%2F1QWTQFMF0Q9JEAGR
    Abstract
    Platelet refractoriness is caused by HLA antibodies and platelet-specific antibodies. Current methods used to detect antiplatelet antibodies have limitations. Solid phase red cell adherence (SPRCA) lacks sensitivity and requires a second assay using chloroquine-treated intact platelets to specify the response due to anti-HLA. We modified SPRCA by using 2 types of antihuman platelet antibodies with different specificities toward platelet lysate and tested samples from 361 patients (69 with unexplained thrombocytopenia and 292 with poor response to platelet transfusions not explicable by alloimmunization or the clinical situation) and 50 from healthy volunteers. Our method compared favorably with platelet suspension direct immunofluorescence. All samples from healthy volunteers were negative; of the samples from the patient population, 240 were positive (147 samples had only antiplatelet and 3 samples had only anti-HLA antibodies). This modified technique had a sensitivity of 98% and a specificity of 91%.;
    Description
    American Journal of Clinical Pathology, 130, 455-66; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Oxford
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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