Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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Distinct Heparan Sulfate Compositions in Wild-Type and Pipe-Mutant Eggshell Matrix, Y. Park, Z. Zhang, R.J. Linhardt, E. K. LeMosy, Fly, 2, 1-5, 2008.
Spatial information embedded in the extracellular matrix establishes the dorsoventral polarity of the Drosophila embryo through the ventral activity of a serine protease cascade. Pipe is a Golgi-localized protein responsible for generating this spatial information during oogenesis through sulfation of unknown glycans. Although Pipe has sequence homology to glycosaminoglycan 2-O-sulfotransferases, its activity and authentic substrates have not been demonstrated and genetic evidence has argued against a role for glycosaminoglycans in dorsoventral polarity establishment. Here, direct examination of matrix glycosaminoglycans demonstrates that pipe-mutant matrix shows decreased tri-sulfated heparan sulfate compared to wild-type matrix, with correspondingly increased 2-O-sulfated heparan sulfate. Chondroitin sulfate was not detected in this matrix. These results suggest that Pipe promotes 6-O- and/or N-sulfation of heparan sulfate but is not required for heparan sulfate 2-O-sulfation. We discuss the possible significance of these unexpected findings and how they might be reconciled with the genetic data.;
Fly, 2, 1-5; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
Taylor and Francis
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Fly; https://harc.rpi.edu/;
Open Access; A full text version is available in DSpace@RPI;