Distinct Heparan Sulfate Compositions in Wild-Type and Pipe-Mutant Eggshell Matrix
Authors
Park, Youmie
Zhang, Zhenqing
Linhardt, Robert J.
LeMosy, Ellen K.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2008-01-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
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Full Citation
Distinct Heparan Sulfate Compositions in Wild-Type and Pipe-Mutant Eggshell Matrix, Y. Park, Z. Zhang, R.J. Linhardt, E. K. LeMosy, Fly, 2, 1-5, 2008.
Abstract
Spatial information embedded in the extracellular matrix establishes the dorsoventral polarity of the Drosophila embryo through the ventral activity of a serine protease cascade. Pipe is a Golgi-localized protein responsible for generating this spatial information during oogenesis through sulfation of unknown glycans. Although Pipe has sequence homology to glycosaminoglycan 2-O-sulfotransferases, its activity and authentic substrates have not been demonstrated and genetic evidence has argued against a role for glycosaminoglycans in dorsoventral polarity establishment. Here, direct examination of matrix glycosaminoglycans demonstrates that pipe-mutant matrix shows decreased tri-sulfated heparan sulfate compared to wild-type matrix, with correspondingly increased 2-O-sulfated heparan sulfate. Chondroitin sulfate was not detected in this matrix. These results suggest that Pipe promotes 6-O- and/or N-sulfation of heparan sulfate but is not required for heparan sulfate 2-O-sulfation. We discuss the possible significance of these unexpected findings and how they might be reconciled with the genetic data.
Description
Fly, 2, 1-5
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Taylor and Francis
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Fly
https://harc.rpi.edu/
Rensselaer Polytechnic Institute, Troy, NY
Fly
https://harc.rpi.edu/
Access
A full text version is available in DSpace@RPI
Open Access
Open Access