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    HSulf-1 modulates FGF-2 and hypoxia mediated migration and invasion of breast cancer cells

    Author
    Khurana, A.; Liu, P.; Mellone, P.; Lorenzon, L.; Vincenzi, B.; Datta, K.; Yang, B.; Linhardt, Robert J.; Lingle, W.; Chien, J.; Baldi, A.; Shridhar, V.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    HSULF-1 MODULATES FGF-2 AND HYPOXIA MEDIATED MIGRATION AND.pdf (1.912Mb)
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    Date Issued
    2011
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    HSulf-1 modulates FGF-2 and hypoxia mediated migration and invasion of breast cancer cells, A. Khurana, P. Liu, P. Mel1one, L. Lorenzon, B. Vincenzi, K. Datta, B. Yang, R. J. Linhardt, W. Lingle, J. Chien, A. Baldi, V. Shridhar, Cancer Research, 71, 2152-2161, 2011.
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    URI
    https://hdl.handle.net/20.500.13015/5240; https://doi.org/10.1158/0008-5472.CAN-10-3059
    Abstract
    HSulf-1 modulates the sulfation states of heparan sulfate proteoglycans critical for heparin binding growth factor signaling. In the present study, we show that HSulf-1 is transcriptionally deregulated under hypoxia in breast cancer cell lines. Knockdown of HIF-1α rescued HSulf-1 downregulation imposed by hypoxia, both at the RNA and protein levels. Chromatin immunoprecipitation with HIF-1α and HIF-2α antibodies confirmed recruitment of HIF-α proteins to the two functional hypoxia-responsive elements on the native HSulf-1 promoter. HSulf-1 depletion in breast cancer cells resulted in an increased and sustained bFGF2 (basic fibroblast growth factor) signaling and promoted cell migration and invasion under hypoxic conditions. In addition, FGFR2 (fibroblast growth factor receptor 2) depletion in HSulf-1-silenced breast cancer cells attenuated hypoxia-mediated cell invasion. Immunohistochemical analysis of 53 invasive ductal carcinomas and their autologous metastatic lesions revealed an inverse correlation for the expression of HSulf-1 to CAIX in both the primary tumors (P ≥ 0.0198) and metastatic lesions (P ≥ 0.0067), respectively, by χ(2) test. Finally, HSulf-1 expression levels in breast tumors by RNA in situ hybridization showed that high HSulf-1 expression is associated with increased disease-free and overall survival (P ≥ 0.03 and P ≥ 0.0001, respectively). Collectively, these results reveal an important link between loss of HSulf-1 under hypoxic microenvironment and increased growth factor signaling, cell migration, and invasion.;
    Description
    Cancer Research, 71, 2152-2161; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    American Association for Cancer Research (AACR)
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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