Analysis of E. coli K5 capsular polysaccharide heparosan
Authors
Ly, Mellisa
Wang, Zhenyu
Laremore, Tatiana N.
Zhang, Fuming
Zhong, Weihong
Pu, Dennis
Zagorevski, Dmitri V.
Dordick, Jonathan S.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2011-01-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
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Full Citation
Analysis of E. coli K5 capsular polysaccharide heparosan, M. Ly, Z. Wang, T. N. Laremore, F. Zhang, W. Zhong, D. Pu, D. V. Zagorevski, J. S. Dordick, R. J. Linhardt, Analytical and Bioanalytical Chemistry, 399, 737-745, 2011.
Abstract
Heparosan is the key precursor for the preparation of bioengineered heparin, a potential replacement for porcine intestinal heparin, an important anticoagulant drug. The molecular weight (MW) distribution of heparosan produced by the fermentation of E. coli K5 was investigated. Large-slab isocratic and mini-slab gradient polyacrylamide gel electrophoresis (PAGE) were used to analyze the MW and polydispersity of heparosan. A preparative method that allowed fractionation by continuous-elution PAGE was used to obtain heparosan MW standards. The MWs of the heparosan standards were determined by electrospray ionization Fourier-transform mass spectrometry (ESI-FT-MS). A ladder of the standards was then used to determine the MW properties of polydisperse heparosan samples. Unbleached and bleached heparosan produced by fermentation of E. coli K5 had similar number-averaged MWs (M(N)), weight-averaged MWs (M(W)), and MW ranges of 3,000 to 150,000 Da.
Description
Analytical and Bioanalytical Chemistry, 399, 737-745
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Springer
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Analytical and Bioanalytical Chemistry
https://harc.rpi.edu/
Rensselaer Polytechnic Institute, Troy, NY
Analytical and Bioanalytical Chemistry
https://harc.rpi.edu/
Access
A full text version is available in DSpace@RPI