Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
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E. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor, Z. Wang, M. Ly, F. Zhang, W. Zhong, A. Suen, J. S. Dordick, R. J. Linhardt, Biotechnology and Bioengineering, 107, 968-977, 2010.
Heparosan is an acidic polysaccharide natural product, which serves as the critical precursor in heparin biosynthesis and in the chemoenzymatic synthesis of bioengineered heparin. Heparosan is also the capsular polysaccharide of Escherichia coli K5 strain. The current study was focused on the examination of the fermentation of E. coli K5 with the goal of producing heparosan in high yield and volumetric productivity. The structure and molecular weight properties of this bacterial heparosan were determined using polyacrylamide gel electrophoresis and Fourier transform-mass spectrometry. Fermentation of E. coli K5 in a defined medium using exponential fed batch glucose addition with oxygen enrichment afforded heparosan at 15 g/L having a number average molecular weight of 58,000 Da and a weight average molecular weight of 84,000 Da.;
Biotechnology and Bioengineering, 107, 968-977; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biotechnology and Bioengineering; https://harc.rpi.edu/;