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    Domain structure elucidation of human decorin glycosaminoglycans

    Author
    Laremore, Tatiana N.; Ly, Mellisa; Zhang, Zhenqing; Solakyildirim, Kemal; McCallum, Scott A.; Owens, Richard T.; Linhardt, Robert J.
    ORCID
    https://orcid.org/0000-0003-2219-5833
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    DOMAIN STRUCTURE ELUCIDATION OF HUMAN DECORIN.pdf (834.9Kb)
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    Date Issued
    2010-10-15
    Subject
    Biology; Chemistry and chemical biology; Chemical and biological engineering; Biomedical engineering
    Degree
    Terms of Use
    In Copyright : this Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). https://rightsstatements.org/page/InC/1.0/;
    Full Citation
    Domain structure elucidation of human decorin glycosaminoglycans, T. N. Laremore, M. Ly, Z. Zhang, K. Solakyildirim, S. McCallum, R. T. Owens, R. J. Linhardt, Biochemical Journal 431, 199-205, 2010.
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    URI
    https://hdl.handle.net/20.500.13015/5248; https://doi.org/10.1042/BJ20100788
    Abstract
    The structure of the GAG (glycosaminoglycan) chain of recombinantly expressed decorin proteoglycan was examined using a combination of intact-chain analysis and domain compositional analysis. The GAG had a number-average molecular mass of 22 kDa as determined by PAGE. NMR spectroscopic analysis using two-dimensional correlation spectroscopy indicated that the ratio of glucuronic acid to iduronic acid in decorin peptidoglycan was 5 to 1. GAG domains terminated with a specific disaccharide obtained by enzymatic degradation of decorin GAG with highly specific endolytic and exolytic lyases were analysed by PAGE and further depolymerized with the enzymes. The disaccharide compositional profiles of the resulting domains were obtained using LC with mass spectrometric and photometric detection and compared with that of the polysaccharide. The information obtained through the disaccharide compositional profiling was combined with the NMR and PAGE data to construct a map of the decorin GAG sequence motifs.;
    Description
    Biochemical Journal 431, 199-205; Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
    Department
    The Linhardt Research Labs.; The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS);
    Publisher
    Portland Press
    Relationships
    The Linhardt Research Labs Online Collection; Rensselaer Polytechnic Institute, Troy, NY; Biochemical Journal; https://harc.rpi.edu/;
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    A full text version is available in DSpace@RPI;
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